PMID- 7475962
OWN - NLM
STAT- MEDLINE
DCOM- 19951220
LR  - 20190701
IS  - 0024-3205 (Print)
IS  - 0024-3205 (Linking)
VI  - 57
IP  - 23
DP  - 1995
TI  - Roles of calcium in the regulation of apoptosis in HL-60 promyelocytic leukemia 
      cells.
PG  - 2091-9
AB  - Increase in intracellular calcium concentrations ([Ca2+]i) is critical for the 
      initiation of apoptosis in cells such as thymocytes and in other cells, calcium 
      chelators may promote apoptosis. However, calcium modulators, such as calcium 
      ionophore 4-bromo-calcium ionophore (Br-A23187) and thapsigargin (TG), induce 
      apoptosis in different cells, including HL-60 cells in which the induction of 
      apoptosis seems a calcium-independent process. These observations imply that the 
      disturbance of calcium homeostasis is probably the most important factor in the 
      regulation of apoptosis. In this article, reagents with different potencies of 
      modulating calcium homeostasis were used to study the possible role of [Ca2+]i 
      and the status of intracellular calcium stores in the causation of HL-60 cell 
      apoptosis. We found that an increase in [Ca2+]i alone did not result in 
      apoptosis, while the depletion of TG-sensitive calcium stores in the endoplasmic 
      reticulum was closely related with the induction of apoptosis. In HL-60 cells, 
      extracellular and intracellular calcium chelators promoted apoptosis. Calmodulin 
      antagonist did not attenuate apoptosis induced by other reagents. Our results 
      suggest that the depletion of Ca2+ stores is an important mean to modulate 
      calcium homeostasis and that the mobilization of calcium (Ca2+) from 
      intracellular stores, rather than an increase in [Ca2+]i, provides the signal for 
      the induction of apoptosis in HL-60 cells.
FAU - Zhu, W H
AU  - Zhu WH
AD  - Department of Physiology, Faculty of Medicine, University of Hong Kong, Hong 
      Kong.
FAU - Loh, T T
AU  - Loh TT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Calmodulin)
RN  - 0 (Terpenes)
RN  - 526U7A2651 (Egtazic Acid)
RN  - 67526-95-8 (Thapsigargin)
RN  - 9007-49-2 (DNA)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - *Apoptosis
MH  - Calcium/*physiology
MH  - Calmodulin/antagonists & inhibitors
MH  - DNA/metabolism
MH  - Egtazic Acid/pharmacology
MH  - HL-60 Cells
MH  - Homeostasis
MH  - Humans
MH  - Terpenes/pharmacology
MH  - Thapsigargin
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
AID - 002432059502202T [pii]
AID - 10.1016/0024-3205(95)02202-t [doi]
PST - ppublish
SO  - Life Sci. 1995;57(23):2091-9. doi: 10.1016/0024-3205(95)02202-t.