PMID- 7479737
OWN - NLM
STAT- MEDLINE
DCOM- 19951130
LR  - 20220411
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 92
IP  - 22
DP  - 1995 Oct 24
TI  - Processing and cryopreservation of placental/umbilical cord blood for unrelated 
      bone marrow reconstitution.
PG  - 10119-22
AB  - Clinical evidence of hematopoietic restoration with placental/umbilical cord 
      blood (PCB) grafts indicates that PCB can be a useful source of hematopoietic 
      stem cells for routine bone marrow reconstitution. In the unrelated setting, 
      human leukocyte antigen (HLA)-matched donors must be obtained for candidate 
      patients and, hence, large panels of frozen HLA-typed PCB units must be 
      established. The large volume of unprocessed units, consisting mostly of red 
      blood cells, plasma, and cryopreservation medium, poses a serious difficulty in 
      this effort because storage space in liquid nitrogen is limited and costly. We 
      report here that almost all the hematopoietic colony-forming cells present in PCB 
      units can be recovered in a uniform volume of 20 ml by using rouleaux formation 
      induced by hydroxyethyl starch and centrifugation to reduce the bulk of 
      erythrocytes and plasma and, thus, concentrate leukocytes. This method multiples 
      the number of units that can be stored in the same freezer space as much as 
      10-fold depending on the format of the storage system. We have also investigated 
      the proportion of functional stem/progenitor cells initially present that are 
      actually available to the recipient when thawed cryopreserved PCB units are 
      infused. Progenitor cell viability is measurably decreased when thawed cells, 
      still suspended in hypertonic cryopreservative solutions, are rapidly mixed with 
      large volumes of isotonic solutions or plasma. The osmotic damage inflicted by 
      the severe solute concentration gradient, however, can be averted by a simple 
      2-fold dilution after thawing, providing almost total recovery of viable 
      hematopoietic progenitor cells.
FAU - Rubinstein, P
AU  - Rubinstein P
AD  - Laboratory of Immunogenetics, Lindsley F. Kimball Research Institute of the New 
      York Blood Center, NY 10021, USA.
FAU - Dobrila, L
AU  - Dobrila L
FAU - Rosenfield, R E
AU  - Rosenfield RE
FAU - Adamson, J W
AU  - Adamson JW
FAU - Migliaccio, G
AU  - Migliaccio G
FAU - Migliaccio, A R
AU  - Migliaccio AR
FAU - Taylor, P E
AU  - Taylor PE
FAU - Stevens, C E
AU  - Stevens CE
LA  - eng
GR  - HL 48031/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (HLA Antigens)
SB  - IM
MH  - Blood Cell Count
MH  - Blood Preservation/*methods
MH  - Blood Sedimentation
MH  - *Bone Marrow
MH  - Cell Separation/methods
MH  - Cell Survival
MH  - Cryopreservation/*methods
MH  - Female
MH  - *Fetal Blood
MH  - HLA Antigens/blood
MH  - Hematopoietic Stem Cells/*cytology
MH  - Histocompatibility Testing
MH  - Humans
MH  - Leukocytes/cytology
MH  - Lymphocytes/cytology
MH  - Neutrophils/cytology
MH  - Placenta
MH  - Pregnancy
PMC - PMC40747
EDAT- 1995/10/24 00:00
MHDA- 1995/10/24 00:01
PMCR- 1996/04/24
CRDT- 1995/10/24 00:00
PHST- 1995/10/24 00:00 [pubmed]
PHST- 1995/10/24 00:01 [medline]
PHST- 1995/10/24 00:00 [entrez]
PHST- 1996/04/24 00:00 [pmc-release]
AID - 10.1073/pnas.92.22.10119 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1995 Oct 24;92(22):10119-22. doi: 
      10.1073/pnas.92.22.10119.