PMID- 7485409
OWN - NLM
STAT- MEDLINE
DCOM- 19951219
LR  - 20211203
IS  - 0002-9440 (Print)
IS  - 1525-2191 (Electronic)
IS  - 0002-9440 (Linking)
VI  - 147
IP  - 5
DP  - 1995 Nov
TI  - Cortical and brainstem-type Lewy bodies are immunoreactive for the 
      cyclin-dependent kinase 5.
PG  - 1465-76
AB  - The immunoreactivity of cortical and brainstem-type Lewy bodies has been 
      investigates with antibodies to the cyclin-dependent kinase 5 (cdk5), to the 
      extracellular regulated kinase 1 (ERK-1), and to the cdc2p34 kinase and with 
      antibodies specific for phosphorylation epitopes typical of paired helical 
      filament-tau (PHF-tau). Both cortical and brainstem-type Lewy bodies in diffuse 
      Lewy body disease and brainstem-type Lewy bodies in Parkinson's disease were 
      found to be immunoreactive for cdk5 but not for cdc2p34 or ERK-1 or with the 
      PHF-tau antibodies. Double immunolabeling showed that cdk5-positive Lewy bodies 
      were also ubiquitin immunoreactive and that cdk5 antibodies labeled as many Lewy 
      bodies as ubiquitin antibodies in adequately fixed tissue. The cdk5 
      immunoreactivity of Lewy bodies was abolished by preabsorption of the antibody 
      with a cdk5 peptide. The antibodies to cdk5 labeled a single 33-kd species on 
      Western blots of human brain homogenates, with a similar intensity in control, 
      diffuse Lewy body disease, and Alzheimer's disease, and this cdk5 species was 
      found mainly in the particulate fraction of brain homogenates. This observation 
      suggests that cdk5 might be a protein kinase involved in the phosphorylation of a 
      molecular component of Lewy bodies, for example, neurofilament proteins known to 
      be present in these inclusions.
FAU - Brion, J P
AU  - Brion JP
AD  - Laboratory of Pathology and Electron Microscopy, Free University of Brussels, 
      Belgium.
FAU - Couck, A M
AU  - Couck AM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (Ubiquitins)
RN  - 0 (tau Proteins)
RN  - EC 2.7.11.1 (Cyclin-Dependent Kinase 5)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - EC 2.7.11.22 (CDC2 Protein Kinase)
RN  - EC 2.7.11.22 (CDK5 protein, human)
RN  - EC 2.7.11.22 (Cyclin-Dependent Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Alzheimer Disease/immunology/pathology
MH  - Antibodies, Monoclonal/chemistry
MH  - Brain Stem/immunology/*pathology
MH  - CDC2 Protein Kinase/immunology
MH  - Calcium-Calmodulin-Dependent Protein Kinases/immunology
MH  - Cerebral Cortex/chemistry/immunology/*pathology
MH  - Cyclin-Dependent Kinase 5
MH  - *Cyclin-Dependent Kinases
MH  - Heat-Shock Proteins/immunology
MH  - Humans
MH  - Lewy Bodies/*immunology
MH  - Middle Aged
MH  - Mitogen-Activated Protein Kinase 3
MH  - *Mitogen-Activated Protein Kinases
MH  - Parkinson Disease/immunology/pathology
MH  - Protein Serine-Threonine Kinases/*immunology
MH  - Ubiquitins/immunology
MH  - tau Proteins/immunology
PMC - PMC1869502
EDAT- 1995/11/01 00:00
MHDA- 1995/11/01 00:01
PMCR- 1996/05/01
CRDT- 1995/11/01 00:00
PHST- 1995/11/01 00:00 [pubmed]
PHST- 1995/11/01 00:01 [medline]
PHST- 1995/11/01 00:00 [entrez]
PHST- 1996/05/01 00:00 [pmc-release]
PST - ppublish
SO  - Am J Pathol. 1995 Nov;147(5):1465-76.