PMID- 7488124
OWN - NLM
STAT- MEDLINE
DCOM- 19951221
LR  - 20211203
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 216
IP  - 1
DP  - 1995 Nov 2
TI  - The liver response to in vivo heat shock involves the activation of MAP kinases 
      and RAF and the tyrosine phosphorylation of Shc proteins.
PG  - 54-61
AB  - We have investigated the mechanisms of signal transduction in the response of 
      liver to heat shock in vivo. By immunoblot experiments we have shown that heat 
      shock decreases the electrophoretic mobility of the 40 and 43 kDa mitogen 
      activated protein kinases (MAPKs) and we have found a significant increase of 
      MAPK activity measured as phosphotransferase capacity of both cytosolic extracts 
      and MAPK immunoprecipitates. To elucidate the signalling pathway which accounts 
      for MAPK activation, we focused our attention on its upstream factors, Raf and 
      Ras. We have shown that, heat shock activates Raf-1 kinase and causes an increase 
      in phosphotyrosine content of the 52 kDa Shc protein accompanied by an increment 
      in the amount of coimmunoprecipitated Grb2. These findings provide the first 
      evidence that the Ras-Raf-MAPK pathway is activated in liver during heat shock in 
      vivo.
FAU - Bendinelli, P
AU  - Bendinelli P
AD  - Istituto di Patologia Generale, Universita degli Studi di Milano, Centro di 
      Studio sulla Patologia Cellulare del CNR, Italy.
FAU - Piccoletti, R
AU  - Piccoletti R
FAU - Maroni, P
AU  - Maroni P
FAU - Bernelli-Zazzera, A
AU  - Bernelli-Zazzera A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Adaptor Proteins, Vesicular Transport)
RN  - 0 (GRB2 Adaptor Protein)
RN  - 0 (Grb2 protein, rat)
RN  - 0 (Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Shc Signaling Adaptor Proteins)
RN  - 0 (Shc1 protein, rat)
RN  - 0 (Src Homology 2 Domain-Containing, Transforming Protein 1)
RN  - 21820-51-9 (Phosphotyrosine)
RN  - EC 2.7.10.1 (ErbB Receptors)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-raf)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
SB  - IM
MH  - *Adaptor Proteins, Signal Transducing
MH  - *Adaptor Proteins, Vesicular Transport
MH  - Animals
MH  - Calcium-Calmodulin-Dependent Protein Kinases/isolation & purification/*metabolism
MH  - Chromatography, Affinity
MH  - Enzyme Activation
MH  - ErbB Receptors/metabolism
MH  - GRB2 Adaptor Protein
MH  - *Hot Temperature
MH  - Immunoblotting
MH  - Liver/enzymology/*physiology
MH  - Male
MH  - Molecular Weight
MH  - Phosphotyrosine/analysis
MH  - Protein Serine-Threonine Kinases/*metabolism
MH  - Proteins/isolation & purification/*metabolism
MH  - Proto-Oncogene Proteins/*metabolism
MH  - Proto-Oncogene Proteins c-raf
MH  - Rats
MH  - Rats, Wistar
MH  - Shc Signaling Adaptor Proteins
MH  - Shock
MH  - *Signal Transduction
MH  - Src Homology 2 Domain-Containing, Transforming Protein 1
EDAT- 1995/11/02 00:00
MHDA- 1995/11/02 00:01
CRDT- 1995/11/02 00:00
PHST- 1995/11/02 00:00 [pubmed]
PHST- 1995/11/02 00:01 [medline]
PHST- 1995/11/02 00:00 [entrez]
AID - S0006291X85725910 [pii]
AID - 10.1006/bbrc.1995.2591 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1995 Nov 2;216(1):54-61. doi: 10.1006/bbrc.1995.2591.