PMID- 7488231
OWN - NLM
STAT- MEDLINE
DCOM- 19951214
LR  - 20190623
IS  - 0006-2952 (Print)
IS  - 0006-2952 (Linking)
VI  - 50
IP  - 8
DP  - 1995 Oct 12
TI  - Modulation by benzo[a]pyrene of epidermal growth factor receptors, cell 
      proliferation, and secretion of human chorionic gonadotropin in human placental 
      cell lines.
PG  - 1171-80
AB  - Clinical observations indicate that maternal cigarette smoking has significant 
      detrimental effects on fetoplacental development. The present study used human 
      trophoblastic choriocarcinoma cell lines of placental origin to investigate the 
      effects of benz[a]pyrene (BaP) on epidermal growth factor (EGF) receptors, cell 
      proliferation and human chorionic gonadotropin (hCG) secretion. BaP decreased 
      125I-EGF binding and EGF receptor protein in a concentration-related manner in 
      both BeWo and JEG-3 cell lines. The steady-state level of EGF receptor mRNA, 
      however, was not changed significantly by BaP in either cell line. Cell 
      proliferation was unchanged or slightly increased following exposure to 10 and 50 
      microM BaP in the presence of serum, whereas proliferation progressively 
      decreased in cells exposed under serum-free conditions. The mitogenic effect of 
      EGF was inhibited by cotreatment with BaP in both cell lines. Further study of 
      trophoblast endocrine function showed that both basal and EGF-stimulated 
      secretion of hCG was reduced significantly by BaP exposure in BeWo cells, whereas 
      no adverse effect was seen in JEG-3 cells. Finally, cytochrome P450 1A1 (CYP1A1) 
      was induced in a concentration-dependent manner by BaP in both cell lines. Thus, 
      data indicate that the BaP-mediated loss of EGF receptors alters trophoblast 
      proliferation and endocrine function, and that different mechanisms may be 
      involved in the regulation of hCG secretion in BeWo and JEG-3 cells. In addition, 
      this study supports the feasibility of using the BeWo and JEG-3 trophoblastic 
      choriocarcinoma cell lines to investigate biomarkers and mechanisms of placental 
      toxicity.
FAU - Zhang, L
AU  - Zhang L
AD  - Department of Pharmacology and Therapeutics, College of Medicine, University of 
      Florida, Gainesville 32610, USA.
FAU - Connor, E E
AU  - Connor EE
FAU - Chegini, N
AU  - Chegini N
FAU - Shiverick, K T
AU  - Shiverick KT
LA  - eng
GR  - ES 04435/ES/NIEHS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Biochem Pharmacol
JT  - Biochemical pharmacology
JID - 0101032
RN  - 0 (Carcinogens)
RN  - 0 (Chorionic Gonadotropin)
RN  - 0 (RNA, Messenger)
RN  - 3417WMA06D (Benzo(a)pyrene)
RN  - 9035-51-2 (Cytochrome P-450 Enzyme System)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Benzo(a)pyrene/*toxicity
MH  - Carcinogens/*toxicity
MH  - Cell Division/drug effects
MH  - Choriocarcinoma
MH  - Chorionic Gonadotropin/*metabolism
MH  - Cytochrome P-450 Enzyme System/biosynthesis
MH  - ErbB Receptors/genetics/*metabolism
MH  - Humans
MH  - Placenta/*drug effects/metabolism
MH  - RNA, Messenger/metabolism
MH  - Tumor Cells, Cultured/drug effects
EDAT- 1995/10/12 00:00
MHDA- 1995/10/12 00:01
CRDT- 1995/10/12 00:00
PHST- 1995/10/12 00:00 [pubmed]
PHST- 1995/10/12 00:01 [medline]
PHST- 1995/10/12 00:00 [entrez]
AID - 000629529500253V [pii]
AID - 10.1016/0006-2952(95)00253-v [doi]
PST - ppublish
SO  - Biochem Pharmacol. 1995 Oct 12;50(8):1171-80. doi: 10.1016/0006-2952(95)00253-v.