PMID- 7488747
OWN - NLM
STAT- MEDLINE
DCOM- 19960102
LR  - 20190718
IS  - 0959-4965 (Print)
IS  - 0959-4965 (Linking)
VI  - 6
IP  - 10
DP  - 1995 Jul 10
TI  - Glutamate regulation of GDNF gene expression in the striatum and primary striatal 
      astrocytes.
PG  - 1454-8
AB  - The aim of this study was to investigate the regulation of glial cell 
      line-derived neurotrophic factor (GDNF) mRNA by activation of glutamate receptors 
      in the rat striatum. We observed an increase in GDNF mRNA levels in the adult rat 
      striatum after administration of subseizure doses of N-methyl-D,L-aspartic acid 
      (NMA) and kainic acid. Since it is unclear whether the upregulation of GDNF 
      occurred in neurons or astrocytes within the striatum, we further investigated 
      whether GDNF gene expression in primary striatal astrocytes in culture could be 
      regulated by glutamate receptor activation. We found that treatment of the 
      cultures with NMA and kainic acid similarly upregulated GDNF gene expression as 
      observed in vivo, suggesting that striatal astrocytes express functional 
      glutamate receptors. Immunocytochemical and nuclease protection analysis revealed 
      that striatal astrocytes expressed the NMDA-R1 subunit. These findings suggest 
      the regulation of GDNF mRNA in the striatum may be mediated by excitation of 
      glutamate receptors via glutamatergic cortical afferents.
FAU - Ho, A
AU  - Ho A
AD  - Fishberg Research Center for Neurobiology, Mount Sinai School of Medicine, New 
      York, NY 10029, USA.
FAU - Gore, A C
AU  - Gore AC
FAU - Weickert, C S
AU  - Weickert CS
FAU - Blum, M
AU  - Blum M
LA  - eng
GR  - AG-08538/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Neuroreport
JT  - Neuroreport
JID - 9100935
RN  - 0 (Excitatory Amino Acid Agonists)
RN  - 0 (Gdnf protein, rat)
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - 6384-92-5 (N-Methylaspartate)
RN  - SIV03811UC (Kainic Acid)
SB  - IM
MH  - Animals
MH  - Astrocytes/drug effects/*metabolism
MH  - Cells, Cultured
MH  - Excitatory Amino Acid Agonists/pharmacology
MH  - Gene Expression Regulation/*physiology
MH  - Glial Cell Line-Derived Neurotrophic Factor
MH  - Glutamic Acid/*physiology
MH  - Immunohistochemistry
MH  - Kainic Acid/pharmacology
MH  - Male
MH  - N-Methylaspartate/pharmacology
MH  - Neostriatum/cytology/drug effects/*metabolism
MH  - Nerve Growth Factors/*biosynthesis/genetics
MH  - Nerve Tissue Proteins/*biosynthesis/genetics
MH  - RNA, Messenger/biosynthesis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, N-Methyl-D-Aspartate/metabolism
EDAT- 1995/07/10 00:00
MHDA- 1995/07/10 00:01
CRDT- 1995/07/10 00:00
PHST- 1995/07/10 00:00 [pubmed]
PHST- 1995/07/10 00:01 [medline]
PHST- 1995/07/10 00:00 [entrez]
AID - 10.1097/00001756-199507100-00023 [doi]
PST - ppublish
SO  - Neuroreport. 1995 Jul 10;6(10):1454-8. doi: 10.1097/00001756-199507100-00023.