PMID- 7488866
OWN - NLM
STAT- MEDLINE
DCOM- 19960104
LR  - 20221012
IS  - 1050-7256 (Print)
IS  - 1050-7256 (Linking)
VI  - 5
IP  - 4
DP  - 1995 Aug
TI  - Effects of monoclonal antibody against CD45RB on peripheral blood mononuclear 
      cell proliferation and on HLA-DR and adhesion molecule expression on thyrocytes 
      of patients with autoimmune thyroid disease.
PG  - 265-72
AB  - To evaluate the role of CD45 (especially that of the ectodomain region B) on 
      immunocyte-thyrocyte signaling in patients with autoimmune thyroid disease 
      (AITD), we have examined the in vitro and in vivo effects of a monoclonal 
      antibody (mAb) against with CD45RB, termed MT3. MT3 was added to cultured 
      peripheral blood mononuclear cells (PBMC) from patients with AITD and was 
      additionally injected into severe combined immunodeficient (SCID) mice to which 
      Graves' thyroid cells and intrathyroidal lymphocytes were engrafted. MT3 
      stimulated proliferation of PBMC when cultured for 2 to 3 days in patients with 
      Hashimoto's thyroiditis (HT) and Graves' disease (GD) and in normal controls 
      (NC). However, when cultured for 7 days, the stimulation index [SI: counts per 
      minute (cpm) with mAb/cpm without mAb] was lowered by MT3 in NC and GD patients. 
      However, the mean SI was not lowered in patients with HT. In SCID mice, the 
      concentrations of human immunoglobulin G, antithyroglobulin and 
      antithyroperoxidase antibodies in sera were not significantly changed by 
      injecting MT3. The expression of human leukocyte antigen (HLA)-DR and 
      intercellular adhesion molecule (ICAM)-1 on engrafted human thyrocytes decreased 
      after the tissues were engrafted into the control mice to which vehicle alone was 
      injected. However, in the mice injected with MT3, HLA-DR and ICAM-1 expression 
      remained high or up-regulated by the injection.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Nishikawa, M
AU  - Nishikawa M
AD  - Endocrinology Research Laboratory, Wellesley Hospital, University of Toronto, 
      Ontario, Canada.
FAU - Mukuta, T
AU  - Mukuta T
FAU - Arreaza, G
AU  - Arreaza G
FAU - Resetkova, E
AU  - Resetkova E
FAU - Poppema, S
AU  - Poppema S
FAU - Tamai, H
AU  - Tamai H
FAU - Volpe, R
AU  - Volpe R
FAU - Lazarovits, A I
AU  - Lazarovits AI
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Thyroid
JT  - Thyroid : official journal of the American Thyroid Association
JID - 9104317
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (HLA-DR Antigens)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - EC 3.1.3.48 (Leukocyte Common Antigens)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/*pharmacology
MH  - Cell Division/*drug effects
MH  - Cells, Cultured
MH  - Graves Disease/*immunology
MH  - HLA-DR Antigens/analysis
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/analysis
MH  - Leukocyte Common Antigens/*immunology
MH  - Leukocytes, Mononuclear/*cytology
MH  - Lymphocyte Activation/drug effects
MH  - Mice
MH  - Mice, SCID
MH  - T-Lymphocytes/chemistry/immunology/transplantation
MH  - Thyroid Gland/chemistry/immunology
MH  - Thyroiditis, Autoimmune/*immunology
EDAT- 1995/08/01 00:00
MHDA- 1995/08/01 00:01
CRDT- 1995/08/01 00:00
PHST- 1995/08/01 00:00 [pubmed]
PHST- 1995/08/01 00:01 [medline]
PHST- 1995/08/01 00:00 [entrez]
AID - 10.1089/thy.1995.5.265 [doi]
PST - ppublish
SO  - Thyroid. 1995 Aug;5(4):265-72. doi: 10.1089/thy.1995.5.265.