PMID- 7490544
OWN - NLM
STAT- MEDLINE
DCOM- 19960104
LR  - 20190709
IS  - 0022-0795 (Print)
IS  - 0022-0795 (Linking)
VI  - 147
IP  - 2
DP  - 1995 Nov
TI  - Effects of maternal diabetes on fetal expression of insulin-like growth factor 
      and insulin-like growth factor binding protein mRNAs in the rat.
PG  - R5-8
AB  - Maternal diabetes is associated in humans and rats with an increased risk for 
      fetal growth abnormalities and malformations. Therefore, the effect of maternal 
      diabetes on expression of genes that regulate fetal growth and differentiation is 
      of considerable interest. Developmental growth is regulated in part by the 
      expression and availability of insulin-like growth factors (IGFs). Postnatal 
      expression of a subset of the IGFs and IGF binding proteins (IGFBPs) has been 
      demonstrated to be regulated in response to diabetes and other metabolic 
      conditions. We used in situ hybridization to analyze the effect of maternal 
      diabetes, induced by streptozotocin (STZ) prior to mating, upon prenatal rat IGF 
      and IGFBP mRNA expression. At gestational day (GD) 14, the most striking effect 
      of maternal diabetes on fetal IGF/IGFBP gene expression was a marked increase in 
      the abundance of IGFBP-1 mRNA within the liver primordia of fetuses isolated from 
      diabetic dams compared to age-matched controls. This upregulation cannot be 
      entirely due to the approximately one-half-day delay in fetal development (based 
      on limb bud staging) associated with maternal diabetes, as there was no gross 
      difference in the level of IGFBP-1 mRNA between GD13 and GD14 control fetal 
      livers. In contrast, the fetal mRNA expression patterns of IGF-I, IGF-II and 
      IGFBP-2, -3, -4, -5 and -6 were not grossly altered by maternal diabetes. These 
      data are consistent with the hypothesis that IGFBP-1 produced within the fetal 
      liver and secreted into fetal circulation may play a role in regulating rat fetal 
      growth.
FAU - Streck, R D
AU  - Streck RD
AD  - Division of Reproductive & Developmental Toxicology, Department of Health and 
      Human Services, Jefferson, AR 72079, USA.
FAU - Rajaratnam, V S
AU  - Rajaratnam VS
FAU - Fishman, R B
AU  - Fishman RB
FAU - Webb, P J
AU  - Webb PJ
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Endocrinol
JT  - The Journal of endocrinology
JID - 0375363
RN  - 0 (Insulin-Like Growth Factor Binding Protein 1)
RN  - 0 (RNA, Messenger)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
SB  - IM
MH  - Animals
MH  - Diabetes Mellitus, Experimental/*metabolism
MH  - Embryonic and Fetal Development
MH  - Female
MH  - Gestational Age
MH  - In Situ Hybridization
MH  - Insulin-Like Growth Factor Binding Protein 1/genetics/*metabolism
MH  - Insulin-Like Growth Factor I/genetics/*metabolism
MH  - Liver/*embryology/metabolism
MH  - Pregnancy
MH  - *Pregnancy in Diabetics
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 1995/11/01 00:00
MHDA- 1995/11/01 00:01
CRDT- 1995/11/01 00:00
PHST- 1995/11/01 00:00 [pubmed]
PHST- 1995/11/01 00:01 [medline]
PHST- 1995/11/01 00:00 [entrez]
AID - 10.1677/joe.0.147r005 [doi]
PST - ppublish
SO  - J Endocrinol. 1995 Nov;147(2):R5-8. doi: 10.1677/joe.0.147r005.