PMID- 7491517
OWN - NLM
STAT- MEDLINE
DCOM- 19960104
LR  - 20211203
IS  - 0039-6060 (Print)
IS  - 0039-6060 (Linking)
VI  - 118
IP  - 6
DP  - 1995 Dec
TI  - Molecular and cytogenetic characterization of a t(1;10;21) translocation in the 
      human papillary thyroid cancer cell line TPC-1 expressing the ret/H4 chimeric 
      transcript.
PG  - 1018-23
AB  - BACKGROUND: Activation of the ret proto-oncogene by three different chromosomal 
      rearrangements occurs in up to 25% of papillary thyroid carcinomas. We developed 
      a rapid screening technique to detect ret rearrangements in human interphase and 
      metaphase cells on the basis of multicolor fluorescence in situ hybridization 
      (FISH) of locus-specific DNA probes. METHODS: DNA from individual clones 
      representing the respective ends of a yeast artificial chromosome (YAC) contig 
      spanning the entire ret gene locus were labeled with either digoxigenin 
      (visualized in red) or biotin (green) and hybridized to normal human lymphocytes 
      and the papillary thyroid cancer cell line TPC-1 expressing the ret/H4 chimeric 
      transcript. Further detailed analysis was performed with whole chromosome 
      painting probes and locus-specific probes (YACs, P1s, DNA repeat probes) on tumor 
      metaphase spreads. RESULTS: Hybridization of the YACs to unrearranged ret loci in 
      normal human lymphocyte interphase nuclei showed two yellow domains because of 
      probe overlap. Hybridization to TPC-1 interphase nuclei showed one yellow domain, 
      and 1 red and 1 green domain separated by a large physical distance. Further 
      analysis of metaphase spreads revealed a complex translocation t(1;10;21)(1pter > 
      1q31::21q22.1 > 21qter; 10q11.2 > 10pter::1q31 > 1qter; 21pter > 21q22.1;;10q21.2 
      > 10q11.2::10q21.2 > 10qter) and loss of the H4 gene locus on the nontranslocated 
      chromosome 10. CONCLUSIONS: Break point spanning probes can reliably detect ret 
      rearrangements in interphase nuclei. Locus-specific and whole chromosome painting 
      probes can be used to further characterize complex rearrangements by fluorescence 
      in situ hybridization to metaphase spreads. The papillary thyroid cancer cell 
      line TPC-1 carries the paracentric inversion 10q, inv(10)(q11.2q21) and a complex 
      t(1; 10; 21) translocation. Deletion of the H4 gene on the chromosome 10 not 
      involved in the t(1; 10; 21) translocation suggests lack of normal H4 expression 
      in the TPC-1 cell line. Further studies will have to address the role of the H4 
      gene product in tumor genesis and progression.
FAU - Jossart, G H
AU  - Jossart GH
AD  - Department of Surgery, UCSF/Mount Zion Medical Center, USA.
FAU - Greulich, K M
AU  - Greulich KM
FAU - Siperstein, A E
AU  - Siperstein AE
FAU - Duh, Q
AU  - Duh Q
FAU - Clark, O H
AU  - Clark OH
FAU - Weier, H U
AU  - Weier HU
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Surgery
JT  - Surgery
JID - 0417347
RN  - 0 (DNA Probes)
RN  - 0 (MAS1 protein, human)
RN  - 0 (Proto-Oncogene Mas)
SB  - IM
MH  - Carcinoma, Papillary/*genetics
MH  - Chimera
MH  - Chromosomes, Artificial, Yeast
MH  - *Chromosomes, Human, Pair 1
MH  - *Chromosomes, Human, Pair 10
MH  - *Chromosomes, Human, Pair 21
MH  - DNA Probes
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Proto-Oncogene Mas
MH  - Thyroid Neoplasms/*genetics
MH  - *Translocation, Genetic
MH  - Tumor Cells, Cultured
EDAT- 1995/12/01 00:00
MHDA- 1995/12/01 00:01
CRDT- 1995/12/01 00:00
PHST- 1995/12/01 00:00 [pubmed]
PHST- 1995/12/01 00:01 [medline]
PHST- 1995/12/01 00:00 [entrez]
AID - S0039-6060(05)80108-4 [pii]
AID - 10.1016/s0039-6060(05)80108-4 [doi]
PST - ppublish
SO  - Surgery. 1995 Dec;118(6):1018-23. doi: 10.1016/s0039-6060(05)80108-4.