PMID- 7491535
OWN - NLM
STAT- MEDLINE
DCOM- 19960104
LR  - 20190913
IS  - 0039-6060 (Print)
IS  - 0039-6060 (Linking)
VI  - 118
IP  - 6
DP  - 1995 Dec
TI  - Hyperparathyroidism of multiple endocrine neoplasia type 1: candidate gene and 
      parathyroid calcium sensing protein expression.
PG  - 924-30; discussion 930-1
AB  - BACKGROUND: Hyperparathyroidism affects most patients with multiple endocrine 
      neoplasia type 1 (MEN 1). This study investigates expression of the candidate 
      MEN1 gene phospholipase C beta 3 (PLC beta 3) and expression and function of a 
      putative calcium sensing protein (CAS) in hyperparathyroidism of MEN 1. METHODS: 
      In 31 parathyroid glands from 17 patients with MEN 1, CAS distribution was 
      studied immunohistochemically and parallel sections were explored for PLC beta 3 
      mRNA expression by in situ hybridization. Enzymatically dispersed parathyroid 
      cells were analyzed for cytoplasmic calcium concentrations [Ca2+]i and 
      parathyroid hormone (PTH) release. RESULTS: All glands exhibited a 
      heterogeneously reduced CAS immunoreactivity, especially meager in nodularly 
      assembled parathyroid cells. Calcium regulated [Ca2+]i and PTH release tended to 
      be more deranged in the glands possessing the lowest immunostaining. Parathyroid 
      PLC beta 3 invariably was homogeneously expressed, and this included even MEN 1 
      patients with reduced PLC beta 3 expression in endocrine pancreatic tumors. 
      CONCLUSIONS: The findings support variable calcium insensitivity of [Ca2+]i and 
      PTH release in hyperparathyroidism of MEN 1, apparently coupled to 
      heterogeneously reduced CAS expression. For clarification of the role of PLC beta 
      3 in MEN 1 parathyroid tumorigenesis further study of this protein is required.
FAU - Carling, T
AU  - Carling T
AD  - Department of Surgery, University Hospital, Uppsala, Sweden.
FAU - Rastad, J
AU  - Rastad J
FAU - Ridefelt, P
AU  - Ridefelt P
FAU - Gobl, A
AU  - Gobl A
FAU - Hellman, P
AU  - Hellman P
FAU - Oberg, K
AU  - Oberg K
FAU - Rask, L
AU  - Rask L
FAU - Larsson, C
AU  - Larsson C
FAU - Juhlin, C
AU  - Juhlin C
FAU - Akerstrom, G
AU  - Akerstrom G
AU  - et al.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Surgery
JT  - Surgery
JID - 0417347
RN  - 0 (Parathyroid Hormone)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Calcium-Sensing)
RN  - 0 (Receptors, Cell Surface)
RN  - EC 3.1.4.- (Type C Phospholipases)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Adult
MH  - Calcium/metabolism
MH  - Female
MH  - *Gene Expression
MH  - Humans
MH  - Hyperparathyroidism/complications/*genetics
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Male
MH  - Middle Aged
MH  - Multiple Endocrine Neoplasia Type 1/complications/*genetics
MH  - Parathyroid Glands/chemistry/metabolism
MH  - Parathyroid Hormone/metabolism
MH  - RNA, Messenger/analysis
MH  - Receptors, Calcium-Sensing
MH  - Receptors, Cell Surface/analysis/*genetics
MH  - Type C Phospholipases/analysis/*genetics
EDAT- 1995/12/01 00:00
MHDA- 1995/12/01 00:01
CRDT- 1995/12/01 00:00
PHST- 1995/12/01 00:00 [pubmed]
PHST- 1995/12/01 00:01 [medline]
PHST- 1995/12/01 00:00 [entrez]
AID - S0039-6060(05)80095-9 [pii]
AID - 10.1016/s0039-6060(05)80095-9 [doi]
PST - ppublish
SO  - Surgery. 1995 Dec;118(6):924-30; discussion 930-1. doi: 
      10.1016/s0039-6060(05)80095-9.