PMID- 7491620
OWN - NLM
STAT- MEDLINE
DCOM- 19951229
LR  - 20190909
IS  - 0040-8166 (Print)
IS  - 0040-8166 (Linking)
VI  - 27
IP  - 5
DP  - 1995 Oct
TI  - Peroxisomes and sterol carrier protein-2 in luteal cell steroidogenesis: a 
      possible role in cholesterol transport from lipid droplets to mitochondria.
PG  - 483-90
AB  - In the present investigation, we have studied peroxisomes and sterol carrier 
      protein-2 (SCP2) in control and luteinizing hormone stimulated rat luteal cells. 
      Superovulated immature rats in mid-luteal phase (8 days after ovulation) were 
      divided into two groups (n = 4/group) and treated with vehicle (0.2 ml saline), 
      or luteinizing hormone (LH, 20 micrograms/rat). In this animal model, LH acutely 
      stimulates steroidogenesis. Thirty minutes later, corpora lutea were fixed by 
      whole body perfusion and processed for (1) electron microscopic 
      immunocytochemistry to localize SCP2 via the protein A gold immunolabeling 
      technique, and for (2) electron microscopic histochemistry to stain peroxisomal 
      catalase via the alkaline 3,3'-diaminobenzidine tetrahydrochloride method. In the 
      steroidogenic, mid-phase luteal cells of vehicle treated rats (controls), SCP2 
      was highly concentrated in peroxisomes and sparsely scattered on mitochondria, 
      but no labeling was observed in lipid droplets. In the luteal cells of rats 
      acutely stimulated with LH, peroxisomes immunolabeled for SCP2 were observed 
      within the luteal cell lipid droplets and mitochondria, and in union with lipid 
      droplets and mitochondria. Moreover, in contrast to control luteal cells, 
      significant immunolabeling for SCP2 was detected within the lipid droplets and 
      mitochondria in luteal cells of LH-treated rats. As SCP2 binds cholesterol to 1:1 
      molar ratio and is known to be involved in the intracellular movement of 
      cholesterol, these findings suggest that peroxisomes and SCP2 may possibly be 
      involved in delivering cholesterol from lipid droplets to the mitochondria when 
      luteal cell steroidogenesis is acutely stimulated by LH.
FAU - Mendis-Handagama, S M
AU  - Mendis-Handagama SM
AD  - Department of Animal Science, College of Veterinary Medicine, University of 
      Tennessee, Knoxville 37996, USA.
FAU - Aten, R F
AU  - Aten RF
FAU - Watkins, P A
AU  - Watkins PA
FAU - Scallen, T J
AU  - Scallen TJ
FAU - Berhman, H R
AU  - Berhman HR
LA  - eng
PT  - Journal Article
PL  - Scotland
TA  - Tissue Cell
JT  - Tissue & cell
JID - 0214745
RN  - 0 (Carrier Proteins)
RN  - 0 (Plant Proteins)
RN  - 0 (Sterols)
RN  - 0 (sterol carrier proteins)
RN  - 97C5T2UQ7J (Cholesterol)
SB  - IM
MH  - Animals
MH  - Biological Transport
MH  - Carrier Proteins/*metabolism
MH  - Cholesterol/*metabolism
MH  - Female
MH  - Lipid Metabolism
MH  - Luteal Cells/*metabolism/ultrastructure
MH  - Microbodies/*metabolism/ultrastructure
MH  - Microscopy, Electron
MH  - Mitochondria/metabolism/ultrastructure
MH  - *Plant Proteins
MH  - Rats
MH  - Sterols/*metabolism
EDAT- 1995/10/01 00:00
MHDA- 1995/10/01 00:01
CRDT- 1995/10/01 00:00
PHST- 1995/10/01 00:00 [pubmed]
PHST- 1995/10/01 00:01 [medline]
PHST- 1995/10/01 00:00 [entrez]
AID - S0040-8166(05)80056-4 [pii]
AID - 10.1016/s0040-8166(05)80056-4 [doi]
PST - ppublish
SO  - Tissue Cell. 1995 Oct;27(5):483-90. doi: 10.1016/s0040-8166(05)80056-4.