PMID- 7492570
OWN - NLM
STAT- MEDLINE
DCOM- 19960111
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1245
IP  - 2
DP  - 1995 Oct 19
TI  - Polymorphonuclear leukocyte chemotaxis induced by zinc, copper and nickel in 
      vitro.
PG  - 145-52
AB  - Metallic dental restorations and prosthetic constructions are susceptible to 
      corrosion in oral environment, resulting in the release of various heavy metal 
      ions. Chloride salts of zinc, copper, nickel, chromium, iron and gold were tested 
      for their ability to promote the migration of polymorphonuclear leukocytes 
      (PMNs). Using a modified Boyden chamber assay for chemotaxis zinc, copper and 
      nickel enhanced the migration of PMN cells in concentration range of 0.5-1.0 mM, 
      whereas no augmentation in migratory activity was noted using chromium or iron. 
      In contrast, an inhibition in migratory activity was observed in cells directed 
      toward gold ions. Exposure of cells to zinc, copper or nickel ions induced an 
      orientation reaction in leukocytes in a similar fashion as the polarization 
      reaction induced by a potent peptide chemoattractant, 
      N-formylmethionylleucylphenylalanine (fMLP), in these cells. Exposure of PMN 
      cells to zinc or nickel in chemotactic concentrations stimulated the chemotaxis 
      of these cells to fMLP 2-fold, whereas pretreatment of the cells with zinc prior 
      to assay markedly decreased the subsequent chemotactic migration of the cells to 
      this metal or to fMLP. The enhanced locomotion of PMN cells induced by zinc, 
      copper or nickel ions was found to be in greater extent due to an increase in 
      directed migration (chemotaxis) rather than an augmentation in random movement 
      (chemokinesis) as assessed by Zigmond-Hirsch checkerboard analysis. These results 
      suggest that zinc, copper and nickel ions attract leukocytes by inducing and 
      promoting the chemotactic response in these cells, which may modulate the 
      inflammatory response of host tissue around such metals.
FAU - Hujanen, E S
AU  - Hujanen ES
AD  - Institute of Dentistry, University of Oulu, Finland.
FAU - Seppa, S T
AU  - Seppa ST
FAU - Virtanen, K
AU  - Virtanen K
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0R0008Q3JB (Chromium)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 789U1901C5 (Copper)
RN  - 7OV03QG267 (Nickel)
RN  - E1UOL152H7 (Iron)
RN  - J41CSQ7QDS (Zinc)
SB  - IM
MH  - Animals
MH  - Cell Adhesion/drug effects
MH  - Cell Polarity/drug effects
MH  - Chemotaxis, Leukocyte/*drug effects
MH  - Chromium/pharmacology
MH  - Copper/*pharmacology
MH  - Female
MH  - Iron/pharmacology
MH  - Microscopy, Electron, Scanning
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophils/*physiology
MH  - Nickel/*pharmacology
MH  - Rats
MH  - Zinc/*pharmacology
EDAT- 1995/10/19 00:00
MHDA- 1995/10/19 00:01
CRDT- 1995/10/19 00:00
PHST- 1995/10/19 00:00 [pubmed]
PHST- 1995/10/19 00:01 [medline]
PHST- 1995/10/19 00:00 [entrez]
AID - 0304-4165(95)00082-M [pii]
AID - 10.1016/0304-4165(95)00082-m [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1995 Oct 19;1245(2):145-52. doi: 
      10.1016/0304-4165(95)00082-m.