PMID- 7496910
OWN - NLM
STAT- MEDLINE
DCOM- 19960116
LR  - 20210526
IS  - 1071-412X (Print)
IS  - 1098-6588 (Electronic)
IS  - 1071-412X (Linking)
VI  - 1
IP  - 1
DP  - 1994 Jan
TI  - Evidence for anti-Plasmodium falciparum antibodies that cross-react with human 
      T-lymphotropic virus type I proteins in a population in Irian Jaya, Indonesia.
PG  - 11-5
AB  - This study was performed to demonstrate the presence of anti-Plasmodium 
      falciparum antibodies in a population living in Irian Jaya, Indonesia that 
      cross-react with human T-lymphotropic virus type I (HTLV-I) proteins. Serum 
      samples from 63 volunteers living in Oksibil, a secluded highland valley in Irian 
      Jaya, were tested for anti-P. falciparum antibodies by an immunofluorescence 
      assay and for anti-HTLV-I antibodies by an enzyme immunoassay (EIA). All samples 
      were positive for anti-P. falciparum antibodies at titers of > or = 1:256. 
      Twenty-four samples were reactive by EIA for HTLV-I, and of these, 23 were tested 
      by western blotting (immunoblotting). Five of the 23 samples were classified as 
      western blot positive and 18 were classified as western blot indeterminate. In 
      competitive blocking assays with malaria proteins, western blot immunoreactivity 
      to all HTLV-I Gag proteins was either reduced or eliminated. Significant 
      reductions in the HTLV-I EIA optical density values of the Oksibil sera occurred 
      when the sera were competitively blocked with the malaria antigens. The optical 
      density values of HTLV-I-positive control sera showed no significant change. 
      Competitive blocking with HTLV-I antigens produced reductions in the optical 
      density values of both the Oksibil sera and the HTLV-I-positive control sera. 
      These data suggest that in this population, anti-P. falciparum antibodies are 
      cross-reactive with HTLV-I proteins in the western blot and EIA tests.
FAU - Porter, K R
AU  - Porter KR
AD  - Infectious Diseases Threat Assessment Program, Naval Medical Research Institute, 
      Bethesda, Maryland 20852, USA.
FAU - Liang, L
AU  - Liang L
FAU - Long, G W
AU  - Long GW
FAU - Bangs, M J
AU  - Bangs MJ
FAU - Anthony, R
AU  - Anthony R
FAU - Andersen, E M
AU  - Andersen EM
FAU - Hayes, C G
AU  - Hayes CG
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Clin Diagn Lab Immunol
JT  - Clinical and diagnostic laboratory immunology
JID - 9421292
RN  - 0 (Antibodies, Protozoan)
RN  - 0 (HTLV-I Antibodies)
RN  - 0 (HTLV-I Antigens)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Animals
MH  - Antibodies, Protozoan/*chemistry
MH  - Binding, Competitive
MH  - Cross Reactions
MH  - HTLV-I Antibodies/chemistry
MH  - HTLV-I Antigens/*immunology
MH  - Humans
MH  - Indonesia/epidemiology
MH  - Malaria, Falciparum/epidemiology/immunology/virology
MH  - Plasmodium falciparum/*immunology
MH  - Viral Proteins/*immunology
PMC - PMC368188
EDAT- 1994/01/01 00:00
MHDA- 1994/01/01 00:01
PMCR- 1994/01/01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1994/01/01 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
PHST- 1994/01/01 00:00 [pmc-release]
AID - 10.1128/cdli.1.1.11-15.1994 [doi]
PST - ppublish
SO  - Clin Diagn Lab Immunol. 1994 Jan;1(1):11-5. doi: 10.1128/cdli.1.1.11-15.1994.