PMID- 7496941
OWN - NLM
STAT- MEDLINE
DCOM- 19960116
LR  - 20210526
IS  - 1071-412X (Print)
IS  - 1098-6588 (Electronic)
IS  - 1071-412X (Linking)
VI  - 1
IP  - 2
DP  - 1994 Mar
TI  - Detection of antibodies to trans-activator protein (p40taxI) of human T-cell 
      lymphotropic virus type I by a synthetic peptide-based assay.
PG  - 176-81
AB  - Antibodies to human T-cell lymphotropic virus type I (HTLV-I) trans-activator 
      protein (p40taxI) were determined in serum specimens from individuals infected 
      with HTLV-I (n = 138) and HTLV-II (n = 19). Western blot (immunoblot) analysis 
      using recombinant tax demonstrated the presence of anti-tax antibodies in 96% of 
      patients (25 of 26) with HTLV-I-associated myelopathy, 43% of those (20 of 46) 
      with adult T-cell leukemia, and 61% of asymptomatic HTLV-I blood donors (40 of 
      66); only one of the HTLV-II specimens reacted with the recombinant tax protein. 
      Synthetic peptides (Tax8(106-125), Tax22(316-335), Tax-23(331-350), and 
      Tax-24(336-353) representing the immunodominant epitopes of p40taxI detected 
      anti-tax antibodies in 66 (48%), 50 (36%), 66 (48%), and 64 (46%) of 138 
      HTLV-I-positive specimens, respectively. An enzyme immunoassay using an equimolar 
      ratio of these four peptides allowed sensitive detection of anti-tax antibodies 
      in 96% of patients (25 of 26) with HTLV-1-associated myelopathy, 52% of adult 
      T-cell leukemia patients (24 of 46), and 62% of asymptomatic HTLV-1-infected 
      donors (41 of 66). The synthetic peptide-based cocktail assay was HTLV-I 
      specific, since none of the HTLV-II-infected specimens reacted with these 
      peptides. Interestingly, the corresponding regions from the HTLV-II tax protein, 
      Tax8II(106-125), and Tax-22II(312-331) did not react with either HTLV-II or 
      HTLV-I specimens. Thus, a synthetic peptide-based assay composed of 
      immunodominant epitopes located towards the amino terminus and the C terminus of 
      p40taxI provides a reliable and sensitive assay for the detection of anti-tax 
      antibodies in seroepidemiologic studies.
FAU - Rudolph, D L
AU  - Rudolph DL
AD  - Retrovirus Diseases Branch, Centers for Disease Control, Atlanta, Georgia 30333, 
      USA.
FAU - Coligan, J E
AU  - Coligan JE
FAU - Lal, R B
AU  - Lal RB
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Clin Diagn Lab Immunol
JT  - Clinical and diagnostic laboratory immunology
JID - 9421292
RN  - 0 (Gene Products, tax)
RN  - 0 (HTLV-I Antibodies)
RN  - 0 (Immunodominant Epitopes)
RN  - 0 (Peptides)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Gene Products, tax/genetics/*immunology
MH  - HTLV-I Antibodies/*blood
MH  - HTLV-I Infections/immunology/virology
MH  - Humans
MH  - Immunodominant Epitopes
MH  - Immunoenzyme Techniques
MH  - Molecular Sequence Data
MH  - Peptides/chemical synthesis/genetics/*immunology
MH  - Recombinant Proteins/*immunology
PMC - PMC368223
EDAT- 1994/03/01 00:00
MHDA- 1994/03/01 00:01
PMCR- 1994/03/01
CRDT- 1994/03/01 00:00
PHST- 1994/03/01 00:00 [pubmed]
PHST- 1994/03/01 00:01 [medline]
PHST- 1994/03/01 00:00 [entrez]
PHST- 1994/03/01 00:00 [pmc-release]
AID - 10.1128/cdli.1.2.176-181.1994 [doi]
PST - ppublish
SO  - Clin Diagn Lab Immunol. 1994 Mar;1(2):176-81. doi: 10.1128/cdli.1.2.176-181.1994.