PMID- 7496962 OWN - NLM STAT- MEDLINE DCOM- 19960116 LR - 20210526 IS - 1071-412X (Print) IS - 1098-6588 (Electronic) IS - 1071-412X (Linking) VI - 1 IP - 3 DP - 1994 May TI - Spontaneous lymphocyte proliferation in human T-cell lymphotropic virus type I (HTLV-I) and HTLV-II infection: T-cell subset responses and their relationships to the presence of provirus and viral antigen production. PG - 273-82 AB - Spontaneous lymphocyte proliferation (SLP) during in vitro culture of mononuclear cells (MCs) characterizes over half of asymptomatic individuals infected with human T-cell lymphotropic virus type I (HTLV-I) or HTLV-II. Both CD4 and CD8 T-cell subsets within MC cultures are activated during SLP, as judged by high-density CD25 (CD25bright) expression; it is unclear, however, whether both cell subsets can directly undergo SLP. In the present investigation, the SLP capacities of purified CD8 and CD4 cells were examined in subjects infected with HTLV-I (n = 19) or HTLV-II (n = 54) in relation to the SLP status of MCs from each subject. No increase in SLP was observed for CD8 or CD4 cells from SLP-negative (SLP-) HTLV-infected subjects, whereas robust SLP characterized CD8 cells from all SLP-positive (SLP+) individuals, regardless of HTLV type. In contrast, SLP+ CD4 cells characterized only 23% (7 of 31) of HTLV-II+ SLP+ individuals, whereas SLP+ CD4 cells characterized 100% of HTLV-I+ SLP+ individuals. In cocultures of HTLV-II+ SLP+ CD8 cells and autologous SLP- CD4 cells, sizable proportions of both CD8 cells and CD4 cells coexpressed CD25bright, suggesting that SLP- CD4 cells were activated in the presence of SLP+ CD8 cells. PCR analysis for tax sequences detected provirus in most CD4- and CD8-cell preparations from HTLV-seropositive individuals, regardless of type and the SLP status of cell subsets. To determine whether SLP was associated with activation of viral genes, levels of HTLV-I and HTLV-II core antigen (Ag) in supernatants were measured. Viral Ag production and SLP responses were significantly correlated for both CD4 and CD8 cells in both HTLV-I and HTLV-II infections. However, inhibition of CD8- or CD4-cell SLP by cyclosporin A or anti-Tac (anti-CD25) did not reduce Ag production, indicating that Ag production is not coupled to SLP. These findings show that CD4 cells from SLP+ HTLV-I+ and SLP+ HTLV-II+ individuals differ in SLP capacity, that the absence of SLP does not indicate a lack of infection, and that production of viral Ag is associated with, but not dependent on, SLP. FAU - Prince, H E AU - Prince HE AD - Cellular Immunology Laboratory, American Red Cross Blood and Tissue Services, Los Angeles, California 90006, USA. FAU - York, J AU - York J FAU - Golding, J AU - Golding J FAU - Owen, S M AU - Owen SM FAU - Lal, R B AU - Lal RB LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Clin Diagn Lab Immunol JT - Clinical and diagnostic laboratory immunology JID - 9421292 RN - 0 (HTLV-I Antigens) RN - 0 (HTLV-II Antigens) SB - IM MH - Base Sequence MH - CD4-Positive T-Lymphocytes/immunology MH - CD8-Positive T-Lymphocytes/immunology MH - Genome, Viral MH - HTLV-I Antigens/*biosynthesis MH - HTLV-I Infections/*immunology MH - HTLV-II Antigens/*biosynthesis MH - HTLV-II Infections/*immunology MH - Humans MH - Leukocytes, Mononuclear/immunology MH - *Lymphocyte Activation MH - Molecular Sequence Data MH - Monocytes/immunology MH - Proviruses/*immunology MH - T-Lymphocyte Subsets/*immunology PMC - PMC368248 EDAT- 1994/05/01 00:00 MHDA- 1994/05/01 00:01 PMCR- 1994/05/01 CRDT- 1994/05/01 00:00 PHST- 1994/05/01 00:00 [pubmed] PHST- 1994/05/01 00:01 [medline] PHST- 1994/05/01 00:00 [entrez] PHST- 1994/05/01 00:00 [pmc-release] AID - 10.1128/cdli.1.3.273-282.1994 [doi] PST - ppublish SO - Clin Diagn Lab Immunol. 1994 May;1(3):273-82. doi: 10.1128/cdli.1.3.273-282.1994.