PMID- 7498534
OWN - NLM
STAT- MEDLINE
DCOM- 19960118
LR  - 20191210
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 376
IP  - 3
DP  - 1995 Dec 4
TI  - Evidence for direct involvement of the sarcoplasmic reticulum Ca(2+)-ATPase in a 
      passive monovalent cation (K+/Na+) exchange.
PG  - 167-71
AB  - A specific inhibitor of SERCA-pumps, thapsigargin (TG) was used to demonstrate 
      the direct involvement of the SR Ca(2+)-ATPase in passive K+/Na+ exchange. The 
      K(+)-potential variations across vesicle membranes were measured in the absence 
      of ATP with a fluorescent probe: 3,3'-dipropylthiodicarbocyanine iodide. Addition 
      of EGTA dissipates the K(+)-potential whereas the presence of TG abolishes this 
      effect. Our data prove that the Ca(2+)-ATPase translocates monovalent cations at 
      a rate similar to the E2-->E1 conformational change.
FAU - de Jesus, F
AU  - de Jesus F
AD  - C.E.A., URA CNRS no. 520, Departement de Biologie Moleculaire et Structurale, 
      Centre d'Etudes Nucleaires de Grenoble, France.
FAU - Cuillel, M
AU  - Cuillel M
FAU - Dupont, Y
AU  - Dupont Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Ionophores)
RN  - 0 (Terpenes)
RN  - 1405-97-6 (Gramicidin)
RN  - 2001-95-8 (Valinomycin)
RN  - 67526-95-8 (Thapsigargin)
RN  - 9NEZ333N27 (Sodium)
RN  - EC 7.2.2.10 (Calcium-Transporting ATPases)
RN  - RWP5GA015D (Potassium)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Biological Transport, Active
MH  - Calcium/*metabolism
MH  - Calcium-Transporting ATPases/antagonists & inhibitors/*metabolism
MH  - Cell-Free System
MH  - Enzyme Inhibitors/pharmacology
MH  - Gramicidin/pharmacology
MH  - Hydrogen-Ion Concentration
MH  - Ionophores/pharmacology
MH  - Membrane Potentials
MH  - Potassium/*metabolism
MH  - Rabbits
MH  - Sarcoplasmic Reticulum/*enzymology
MH  - Sodium/*metabolism
MH  - Terpenes/pharmacology
MH  - Thapsigargin
MH  - Valinomycin/pharmacology
EDAT- 1995/12/04 00:00
MHDA- 1995/12/04 00:01
CRDT- 1995/12/04 00:00
PHST- 1995/12/04 00:00 [pubmed]
PHST- 1995/12/04 00:01 [medline]
PHST- 1995/12/04 00:00 [entrez]
AID - 0014-5793(95)01267-1 [pii]
AID - 10.1016/0014-5793(95)01267-1 [doi]
PST - ppublish
SO  - FEBS Lett. 1995 Dec 4;376(3):167-71. doi: 10.1016/0014-5793(95)01267-1.