PMID- 7499247
OWN - NLM
STAT- MEDLINE
DCOM- 19960117
LR  - 20211203
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 270
IP  - 46
DP  - 1995 Nov 17
TI  - Hepatocyte growth factor-induced scatter of Madin-Darby canine kidney cells 
      requires phosphatidylinositol 3-kinase.
PG  - 27780-7
AB  - Hepatocyte growth factor/scatter factor (HGF/SF) is a multifunctional cytokine 
      that induces mitogenesis, motility, invasion, and morphogenesis of several 
      epithelial and endothelial cell lines in culture. The receptor for HGF/SF has 
      been identified as the Met tyrosine kinase. To investigate the signaling pathways 
      that are involved in these events, we have generated chimeric receptors 
      containing the extracellular domain of the colony-stimulating factor-1 (CSF-1) 
      receptor fused to the transmembrane and intracellular domains of the Met receptor 
      (MET). Madin-Darby canine kidney (MDCK) epithelial cells expressing the CSF-MET 
      chimera dissociate and scatter in response to CSF-1. However, cells expressing a 
      mutant CSF-MET receptor containing a phenylalanine substitution for tyrosine 1356 
      were unable to scatter or form branching tubules following stimulation with 
      CSF-1. Tyrosine 1356 is essential for the recruitment of multiple substrates 
      including the p85 subunit of PI3-kinase, phospholipase C gamma, and Grb2. In this 
      study, we have investigated the role of PI3-kinase and a downstream target of 
      PI3-kinase, pp70S6K, in the induction of MDCK cell scatter in response to HGF/SF. 
      Our results demonstrate that following stimulation with HGF/SF, activation of 
      PI3-kinase but not pp70S6K is essential for MDCK cell scatter.
FAU - Royal, I
AU  - Royal I
AD  - Molecular Oncology Laboratory, Royal Victoria Hospital, Montreal, Quebec, Canada.
FAU - Park, M
AU  - Park M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Androstadienes)
RN  - 0 (Cadherins)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (Desmoplakins)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Peptide Fragments)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - 81627-83-0 (Macrophage Colony-Stimulating Factor)
RN  - EC 2.7.1.- (Phosphotransferases (Alcohol Group Acceptor))
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
RN  - EC 2.7.10.1 (Receptor, Macrophage Colony-Stimulating Factor)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases)
RN  - XVA4O219QW (Wortmannin)
SB  - IM
MH  - Amino Acid Sequence
MH  - Androstadienes/pharmacology
MH  - Animals
MH  - Blotting, Western
MH  - Cadherins/analysis/metabolism
MH  - Cell Adhesion Molecules/analysis/metabolism
MH  - Cell Line
MH  - Cell Movement/drug effects/*physiology
MH  - Cytoskeletal Proteins/analysis/metabolism
MH  - Desmoplakins
MH  - Dogs
MH  - Enzyme Activation
MH  - Enzyme Inhibitors/pharmacology
MH  - Hepatocyte Growth Factor/*pharmacology
MH  - Kidney
MH  - Macrophage Colony-Stimulating Factor/pharmacology
MH  - Molecular Sequence Data
MH  - Peptide Fragments/chemistry
MH  - Phosphatidylinositol 3-Kinases
MH  - Phosphotransferases (Alcohol Group Acceptor)/*metabolism
MH  - Protein Serine-Threonine Kinases/isolation & purification/metabolism
MH  - Proto-Oncogene Proteins c-met
MH  - Receptor Protein-Tyrosine Kinases/biosynthesis/*physiology
MH  - Receptor, Macrophage Colony-Stimulating Factor/biosynthesis/metabolism
MH  - Recombinant Fusion Proteins/biosynthesis/metabolism
MH  - Ribosomal Protein S6 Kinases
MH  - Signal Transduction
MH  - Substrate Specificity
MH  - Wortmannin
EDAT- 1995/11/17 00:00
MHDA- 1995/11/17 00:01
CRDT- 1995/11/17 00:00
PHST- 1995/11/17 00:00 [pubmed]
PHST- 1995/11/17 00:01 [medline]
PHST- 1995/11/17 00:00 [entrez]
AID - S0021-9258(18)88021-8 [pii]
AID - 10.1074/jbc.270.46.27780 [doi]
PST - ppublish
SO  - J Biol Chem. 1995 Nov 17;270(46):27780-7. doi: 10.1074/jbc.270.46.27780.