PMID- 7504523
OWN - NLM
STAT- MEDLINE
DCOM- 19940113
LR  - 20191023
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 8
IP  - 2
DP  - 1993 Oct
TI  - In situ hybridization to interphase nuclei in acute leukemia.
PG  - 98-103
AB  - Numerical chromosome abnormalities were studied in 17 acute lymphoblastic 
      leukemias and one hyperdiploid acute myeloblastic leukemia by fluorescence in 
      situ hybridization (FISH) using YAC clones specific to chromosomes 21 and 6. The 
      results agreed well with cytogenetic findings. Hyperdiploid leukemias with more 
      than 50 chromosomes usually had 4 copies of chromosome 21 and three of chromosome 
      6, while diploid and pseudodiploid cases were confirmed to have two copies of the 
      two chromosomes. Interesting discrepancies were also observed. In one patient, 
      trisomy 6 was detected by FISH but not by cytogenetics because of the probable 
      inclusion of a chromosome 6 segment within a marker chromosome. The percentages 
      of nuclei with 3 or 4 spots (chromosome 21) and three spots (chromosome 6) in 
      hyperdiploid cells were significantly different in some patients, whereas they 
      might be identical from cytogenetic data.
FAU - Romana, S P
AU  - Romana SP
AD  - INSERM U 301, Institut de Genetique Moleculaire, Paris, France.
FAU - Cherif, D
AU  - Cherif D
FAU - Le Coniat, M
AU  - Le Coniat M
FAU - Derre, J
AU  - Derre J
FAU - Flexor, M A
AU  - Flexor MA
FAU - Berger, R
AU  - Berger R
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (DNA Primers)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Base Sequence
MH  - Child
MH  - Child, Preschool
MH  - *Chromosome Aberrations
MH  - Chromosomes, Artificial, Yeast
MH  - *Chromosomes, Human, Pair 21
MH  - *Chromosomes, Human, Pair 6
MH  - DNA Primers/chemistry
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infant
MH  - Interphase
MH  - Karyotyping
MH  - Leukemia, Myeloid, Acute/*genetics
MH  - Male
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*genetics
EDAT- 1993/10/01 00:00
MHDA- 1993/10/01 00:01
CRDT- 1993/10/01 00:00
PHST- 1993/10/01 00:00 [pubmed]
PHST- 1993/10/01 00:01 [medline]
PHST- 1993/10/01 00:00 [entrez]
AID - 10.1002/gcc.2870080206 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 1993 Oct;8(2):98-103. doi: 10.1002/gcc.2870080206.