PMID- 7504889
OWN - NLM
STAT- MEDLINE
DCOM- 19940111
LR  - 20181113
IS  - 0002-9440 (Print)
IS  - 1525-2191 (Electronic)
IS  - 0002-9440 (Linking)
VI  - 143
IP  - 6
DP  - 1993 Dec
TI  - Tumor necrosis factor activates human endothelial cells through the p55 tumor 
      necrosis factor receptor but the p75 receptor contributes to activation at low 
      tumor necrosis factor concentration.
PG  - 1724-30
AB  - Tumor necrosis factor-alpha (TNF-alpha) interacts with two distinct membrane 
      receptor proteins, p55 and p75, which are variably expressed on different cell 
      types. We have examined the function of p55 and p75 on human endothelial cells 
      (EC). Both receptor types are detected on cultured EC by FACS analysis. A 
      mutagenized recombinant human TNF (R32W-TNF), which binds selectively to p55, is 
      equipotent with human recombinant wild-type TNF (wt-TNF) in upregulating several 
      different leukocyte adhesion molecules as well as class I major 
      histocompatibility complex molecules. R32W-TNF also fully desensitizes EC to 
      wt-TNF, as assessed by inhibition of re-induction of endothelial leukocyte 
      adhesion molecule-1 (ELAM-1). At low wt-TNF concentrations, induction of ELAM-1 
      is partly inhibited by blocking monoclonal antibodies to either p55 or p75 and to 
      a greater extent by a combination of both monoclonal antibodies. In contrast, 
      ELAM-1 induction by R32W-TNF is only inhibited by anti-p55. We conclude that both 
      TNF receptors (p55 and p75) can contribute to TNF-induced activation of EC, but 
      that signaling through p55 is sufficient.
FAU - Slowik, M R
AU  - Slowik MR
AD  - Boyer Center for Molecular Medicine, Yale University School of Medicine, New 
      Haven, Connecticut 06536-0812.
FAU - De Luca, L G
AU  - De Luca LG
FAU - Fiers, W
AU  - Fiers W
FAU - Pober, J S
AU  - Pober JS
LA  - eng
GR  - R37-HL-36003/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (E-Selectin)
RN  - 0 (Iodine Radioisotopes)
RN  - 0 (Receptors, Tumor Necrosis Factor)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Antibodies, Monoclonal
MH  - Cell Adhesion Molecules/analysis/physiology
MH  - Cells, Cultured
MH  - Dose-Response Relationship, Drug
MH  - E-Selectin
MH  - Endothelium, Vascular/*chemistry/*cytology/metabolism
MH  - Flow Cytometry
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - Iodine Radioisotopes
MH  - Molecular Weight
MH  - Precipitin Tests
MH  - Receptors, Tumor Necrosis Factor/*analysis/chemistry/*physiology
MH  - Tumor Necrosis Factor-alpha/analysis/metabolism/*pharmacology
PMC - PMC1887273
EDAT- 1993/12/01 00:00
MHDA- 1993/12/01 00:01
PMCR- 1994/06/01
CRDT- 1993/12/01 00:00
PHST- 1993/12/01 00:00 [pubmed]
PHST- 1993/12/01 00:01 [medline]
PHST- 1993/12/01 00:00 [entrez]
PHST- 1994/06/01 00:00 [pmc-release]
PST - ppublish
SO  - Am J Pathol. 1993 Dec;143(6):1724-30.