PMID- 7505064
OWN - NLM
STAT- MEDLINE
DCOM- 19940119
LR  - 20171116
IS  - 0340-6245 (Print)
IS  - 0340-6245 (Linking)
VI  - 70
IP  - 3
DP  - 1993 Sep 1
TI  - Cloning of the cDNA encoding human platelet CD36: comparison to PCR amplified 
      fragments of monocyte, endothelial and HEL cells.
PG  - 500-5
AB  - Glycoprotein CD36, also known as GPIIIb or GPIV, is a major platelet glycoprotein 
      that bears the newly identified Naka alloantigen. The aim of this study was to 
      clone platelet CD36 and investigate other forms of CD36-cDNA present in 
      monocytes, endothelial and HEL cells. RNA from above mentioned cells were reverse 
      transcribed (RT), using specific primers for CD36, and amplified by the 
      polymerase chain reaction (PCR) technique. Sequencing the different amplified 
      platelet derived cDNA fragments, spanning the whole coding and flanking regions, 
      showed the near identity between platelet and CD36-placenta cDNA. Platelet 
      CD36-cDNA cross-hybridized, in Southern blots, with RT-PCR amplified cDNA 
      originating from monocytes, endothelial and HEL cells. However, monocytes showed 
      a RT-PCR amplified cDNA fragment (561 bp) that was present in platelets and 
      placenta but not on endothelial on HEL-cells. Northern blot analysis of platelet 
      RNA hybridized with placenta CD36 indicated the presence of a major (1.95 kb) and 
      a minor (0.95 kb) transcript. The 1.95 kb transcript was the only one observed on 
      Northern blots of monocytes, endothelial and HEL cells. These results indicate 
      that the structure of CD36 expressed in platelets is similar, with the exception 
      of the 3' flanking region, to that of placenta. Differences in apparent molecular 
      weight between CD36 and CD36-like glycoproteins may be due to post-translational 
      modifications.
FAU - Wyler, B
AU  - Wyler B
AD  - INSERM unite 331, Faculte de Medecine Alexis Carrel, Institut Pasteur de Lyon, 
      France.
FAU - Daviet, L
AU  - Daviet L
FAU - Bortkiewicz, H
AU  - Bortkiewicz H
FAU - Bordet, J C
AU  - Bordet JC
FAU - McGregor, J L
AU  - McGregor JL
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Thromb Haemost
JT  - Thrombosis and haemostasis
JID - 7608063
RN  - 0 (Antigens, CD)
RN  - 0 (CD36 Antigens)
RN  - 0 (DNA, Complementary)
RN  - 0 (Platelet Membrane Glycoproteins)
SB  - IM
MH  - Antigens, CD/*blood
MH  - Base Sequence
MH  - CD36 Antigens
MH  - Cloning, Molecular
MH  - DNA, Complementary/*genetics
MH  - Endothelium, Vascular/*immunology
MH  - *Genetic Code
MH  - Humans
MH  - Molecular Sequence Data
MH  - Monocytes/*immunology
MH  - Platelet Membrane Glycoproteins/*immunology
MH  - Polymerase Chain Reaction
MH  - Tumor Cells, Cultured
EDAT- 1993/09/01 00:00
MHDA- 1993/09/01 00:01
CRDT- 1993/09/01 00:00
PHST- 1993/09/01 00:00 [pubmed]
PHST- 1993/09/01 00:01 [medline]
PHST- 1993/09/01 00:00 [entrez]
PST - ppublish
SO  - Thromb Haemost. 1993 Sep 1;70(3):500-5.