PMID- 7506028
OWN - NLM
STAT- MEDLINE
DCOM- 19940131
LR  - 20190623
IS  - 0006-2952 (Print)
IS  - 0006-2952 (Linking)
VI  - 46
IP  - 12
DP  - 1993 Dec 14
TI  - Quercetin-induced expression of rat mast cell protease II and accumulation of 
      secretory granules in rat basophilic leukemia cells.
PG  - 2315-26
AB  - Rat basophilic leukemia (RBL) cells are considered to be similar to bone-marrow 
      derived mast cells and to mucosal mast cells (MMC), the latter of which may be 
      involved in inflammatory bowel diseases. RBL cells are not able to accumulate 
      histamine and secretory granules under regular growing conditions. Here we show 
      that the flavonoid quercetin, which inhibits mast cell secretion of histamine, 
      also inhibited RBL cell proliferation and constitutive histamine release while it 
      induced synthesis of rat mast cell protease (RMCP) II and triggered processes 
      leading to accumulation of secretory granules. Cell viability was also retained 
      in the presence of quercetin, whereas untreated cells did not survive past 6 days 
      of growth. Quercetin did not affect the expression of mRNA for alpha-subunit of 
      immunoglobulin E (IgE) receptor, but led to increased expression of mRNA for, and 
      synthesis of RMCP II, which is a marker protein for MMC. Many of these granules 
      showed metachromasia with toluidine blue after 3 days of growth, stained red with 
      alcian blue counterstained with safranin after 8 days of growth, and contained 
      electron dense material. Our results suggest that RBL cells have the capacity to 
      progress to a more mature state and may lend themselves to further analysis of a 
      growth regulator(s) with action similar to that of quercetin.
FAU - Trnovsky, J
AU  - Trnovsky J
AD  - Department of Pharmacology and Experimental Therapeutics, Tufts University School 
      of Medicine, Boston, MA 02111.
FAU - Letourneau, R
AU  - Letourneau R
FAU - Haggag, E
AU  - Haggag E
FAU - Boucher, W
AU  - Boucher W
FAU - Theoharides, T C
AU  - Theoharides TC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biochem Pharmacol
JT  - Biochemical pharmacology
JID - 0101032
RN  - 0 (RNA, Neoplasm)
RN  - 820484N8I3 (Histamine)
RN  - 9IKM0I5T1E (Quercetin)
RN  - EC 3.4.24.- (Metalloendopeptidases)
RN  - EC 3.4.24.29 (auR protein, Staphylococcus aureus)
SB  - IM
EIN - Biochem Pharmacol 1994 Mar 2;47(5):929
MH  - Animals
MH  - Base Sequence
MH  - Basophils
MH  - Cell Division/drug effects
MH  - Cell Survival/drug effects
MH  - *Cytoplasmic Granules
MH  - Enzyme Induction
MH  - Histamine/biosynthesis
MH  - Histamine Release/drug effects
MH  - Leukemia, Basophilic, Acute
MH  - Mast Cells/drug effects/*enzymology/metabolism
MH  - Metalloendopeptidases/*biosynthesis
MH  - Microscopy, Electron
MH  - Molecular Sequence Data
MH  - Quercetin/*pharmacology
MH  - RNA, Neoplasm/biosynthesis
MH  - Rats
MH  - Tumor Cells, Cultured
EDAT- 1993/12/14 00:00
MHDA- 1993/12/14 00:01
CRDT- 1993/12/14 00:00
PHST- 1993/12/14 00:00 [pubmed]
PHST- 1993/12/14 00:01 [medline]
PHST- 1993/12/14 00:00 [entrez]
AID - 0006-2952(93)90623-5 [pii]
AID - 10.1016/0006-2952(93)90623-5 [doi]
PST - ppublish
SO  - Biochem Pharmacol. 1993 Dec 14;46(12):2315-26. doi: 10.1016/0006-2952(93)90623-5.