PMID- 7506523
OWN - NLM
STAT- MEDLINE
DCOM- 19940210
LR  - 20190717
IS  - 0003-9969 (Print)
IS  - 0003-9969 (Linking)
VI  - 38
IP  - 10
DP  - 1993 Oct
TI  - Effects of hormones and cytokines on stimulation of adenylate cyclase and 
      intracellular calcium concentration in human and canine periodontal-ligament 
      fibroblasts.
PG  - 871-9
AB  - Adenylate cyclase was stimulated by prostaglandin E2 (PGE2) and parathyroid 
      hormone-related protein (PTHrP) in both these types of fibroblast and by 
      calcitonin gene-related protein (CGRP) in the human fibroblasts in vitro. PGE2 (1 
      microM), CGRP (1 microM), and PTHrP (1 microM) stimulated adenylate cyclase up to 
      50-fold, 10-fold and 9-fold, respectively. Calcitonin (CT), substance P (SP), 
      interleukin-1 beta (IL-1 beta), and transforming growth factor-beta 1 (TGF beta 
      1) had no effect on adenylate cyclase in either fibroblast. Intracellular Ca2+ 
      (iCa2+) was measured in individual fibroblasts from the periodontal ligament 
      using Indo-1 and an adherent cell analysis and sorting interactive laser 
      cytometer. Ionomycin (3 microM) caused a transient rise of iCa2+ in all human and 
      canine fibroblasts tested. The mean percentage increase in iCa2+ in response to 
      ionomycin was 820 and 840% for human and canine fibroblasts, respectively. The 
      human fibroblasts responded to PGE2 (1 microM) by an increased iCa2+ 
      concentration; the mean percentage increase in iCa2+ was 187%. SP caused a less 
      pronounced increase in iCa2+ in the human fibroblasts (56%). CGRP and SP caused a 
      similar response in the canine fibroblasts. The mean percentage increase in iCa2+ 
      in response to SP and CGRP was 95 and 78%, respectively. PTH, PTHrP, 
      platelet-activating factor, CT, and IL-1 beta had no effect on iCa2+ in either 
      type of fibroblast. The data indicate that cAMP and calcium have roles as 
      intracellular secondary messengers in the action of PGE2, SP, CGRP, and PTHrP in 
      fibroblasts of human and canine periodontal ligament.
FAU - Nohutcu, R M
AU  - Nohutcu RM
AD  - Department of Veterinary Pathobiology, College of Veterinary Medicine, Ohio State 
      University, Columbus 43210.
FAU - McCauley, L K
AU  - McCauley LK
FAU - Horton, J E
AU  - Horton JE
FAU - Capen, C C
AU  - Capen CC
FAU - Rosol, T J
AU  - Rosol TJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Arch Oral Biol
JT  - Archives of oral biology
JID - 0116711
RN  - 0 (Carrier Proteins)
RN  - 0 (Cyclic AMP Receptor Protein)
RN  - 0 (Cytokines)
RN  - 0 (Hormones)
RN  - 0 (Interleukin-1)
RN  - 0 (PTHLH protein, human)
RN  - 0 (Parathyroid Hormone)
RN  - 0 (Parathyroid Hormone-Related Protein)
RN  - 0 (Platelet Activating Factor)
RN  - 0 (Proteins)
RN  - 0 (Transforming Growth Factor beta)
RN  - 33507-63-0 (Substance P)
RN  - 9007-12-9 (Calcitonin)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 4.6.1.1 (Adenylyl Cyclases)
RN  - JHB2QIZ69Z (Calcitonin Gene-Related Peptide)
RN  - K7Q1JQR04M (Dinoprostone)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Adenylyl Cyclases/analysis/*drug effects
MH  - Adult
MH  - Animals
MH  - Calcitonin/pharmacology
MH  - Calcitonin Gene-Related Peptide/pharmacology
MH  - Calcium/*analysis
MH  - Carrier Proteins
MH  - Cells, Cultured
MH  - Cyclic AMP/analysis
MH  - Cyclic AMP Receptor Protein/analysis
MH  - Cytokines/*pharmacology
MH  - Dinoprostone/pharmacology
MH  - Dogs
MH  - Fibroblasts/cytology/drug effects
MH  - Hormones/*pharmacology
MH  - Humans
MH  - Interleukin-1/pharmacology
MH  - Parathyroid Hormone/pharmacology
MH  - Parathyroid Hormone-Related Protein
MH  - Periodontal Ligament/*chemistry/cytology/*drug effects
MH  - Platelet Activating Factor/pharmacology
MH  - Proteins/pharmacology
MH  - Stimulation, Chemical
MH  - Substance P/pharmacology
MH  - Transforming Growth Factor beta/pharmacology
EDAT- 1993/10/01 00:00
MHDA- 1993/10/01 00:01
CRDT- 1993/10/01 00:00
PHST- 1993/10/01 00:00 [pubmed]
PHST- 1993/10/01 00:01 [medline]
PHST- 1993/10/01 00:00 [entrez]
AID - 0003-9969(93)90096-5 [pii]
AID - 10.1016/0003-9969(93)90096-5 [doi]
PST - ppublish
SO  - Arch Oral Biol. 1993 Oct;38(10):871-9. doi: 10.1016/0003-9969(93)90096-5.