PMID- 7506936
OWN - NLM
STAT- MEDLINE
DCOM- 19940223
LR  - 20190821
IS  - 0300-9084 (Print)
IS  - 0300-9084 (Linking)
VI  - 75
IP  - 8
DP  - 1993
TI  - Serological relationships and epitope profiles of isolates of okra leaf curl 
      geminivirus from Africa and the Middle East.
PG  - 707-11
AB  - A panel of seven murine monoclonal antibodies (mAbs) was raised against particles 
      of okra leaf curl virus (OLCV), a whitefly-transmitted geminivirus which is 
      prevalent in West Africa. The mAbs detected at least six distinguishable 
      epitopes, two continuous and four discontinuous. In tests with these mAbs, 
      relatively little antigenic variation was found among 24 OLCV isolates from 
      Burkina Faso, Chad, Ghana, Ivory Coast, Nigeria, Oman and Saudi Arabia, each 
      virus isolate reacting with at least five of them. However, on the basis of their 
      reactions with 17 mAbs raised against particles of a group A isolate of African 
      cassava mosaic virus (ACMV), the OLCV isolates could be assigned to two groups, 
      which have geographical distributions that overlap in West Africa. A network of 
      antigenic relationships was revealed by reactions between the OLCV mAbs and other 
      whitefly-transmitted geminiviruses from six plant species in 12 countries. OLCV 
      shared the most epitopes with tobacco leaf curl (African isolates) virus, ACMV, 
      Indian cassava mosaic virus and bean golden mosaic virus. Selected OLCV mAbs are 
      suitable for routine detection of OLCV, and a panel of three mAbs can be used to 
      identify it.
FAU - Swanson, M M
AU  - Swanson MM
AD  - Scottish Crop Research Institute Invergowrie, Dundee, UK.
FAU - Harrison, B D
AU  - Harrison BD
LA  - eng
PT  - Journal Article
PL  - France
TA  - Biochimie
JT  - Biochimie
JID - 1264604
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Epitopes)
SB  - IM
MH  - Africa, Western
MH  - Animals
MH  - Antibodies, Monoclonal/immunology
MH  - Antigenic Variation
MH  - Diptera/microbiology
MH  - Epitopes/*immunology
MH  - Geminiviridae/*immunology/isolation & purification
MH  - Insect Vectors
MH  - Mice
MH  - Middle East
MH  - Plant Diseases/microbiology
EDAT- 1993/01/01 00:00
MHDA- 1993/01/01 00:01
CRDT- 1993/01/01 00:00
PHST- 1993/01/01 00:00 [pubmed]
PHST- 1993/01/01 00:01 [medline]
PHST- 1993/01/01 00:00 [entrez]
AID - 0300-9084(93)90101-W [pii]
AID - 10.1016/0300-9084(93)90101-w [doi]
PST - ppublish
SO  - Biochimie. 1993;75(8):707-11. doi: 10.1016/0300-9084(93)90101-w.