PMID- 7507074
OWN - NLM
STAT- MEDLINE
DCOM- 19940223
LR  - 20190516
IS  - 0890-9369 (Print)
IS  - 0890-9369 (Linking)
VI  - 8
IP  - 1
DP  - 1994 Jan
TI  - Activation of the c-Src tyrosine kinase is required for the induction of mammary 
      tumors in transgenic mice.
PG  - 23-32
AB  - Transgenic mice expressing the polyomavirus (PyV) middle T oncogene in the 
      mammary epithelium develop multifocal mammary tumors that metastasize with high 
      frequency. The potent transforming activity of PyV middle T antigen can, in part, 
      be attributed to its ability to associate with and to activate a number of c-Src 
      family tyrosine kinases (c-Src, c-Yes, and Fyn). As a first step toward assessing 
      the role of individual c-Src family tyrosine kinases in PyV middle T 
      antigen-induced mammary tumorigenesis, we have crossed transgenic mice carrying 
      the mouse mammary tumor virus (MMTV)/PyV middle T antigen fusion gene with mice 
      bearing a disrupted c-src proto-oncogene. In contrast to the rapid tumor 
      progression seen in the original MMTV/PyV middle T antigen strains, mice 
      expressing the transgene in the absence of functional c-Src rarely developed 
      mammary tumors. After long latency, these mice did eventually develop abnormal 
      hyperplastic mammary tissue. This growth disturbance was correlated with elevated 
      expression of the PyV middle T antigen and the activation of the PyV middle T 
      antigen-associated c-Yes tyrosine kinase. However, transgenic mice expressing the 
      PyV middle T antigen in the mammary epithelium of wild-type or Yes-deficient mice 
      developed multifocal mammary tumors with comparable kinetics. Taken together, 
      these findings suggest that c-Src tyrosine kinase activity is required for PyV 
      middle T antigen-induced mammary tumorigenesis and also illustrate an in vivo 
      genetic approach to the dissection of mitogenic signal transduction pathways.
FAU - Guy, C T
AU  - Guy CT
AD  - Institute for Molecular Biology and Biotechnology, McMaster University, Hamilton, 
      Ontario, Canada.
FAU - Muthuswamy, S K
AU  - Muthuswamy SK
FAU - Cardiff, R D
AU  - Cardiff RD
FAU - Soriano, P
AU  - Soriano P
FAU - Muller, W J
AU  - Muller WJ
LA  - eng
GR  - R01-CA-S4285/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genes Dev
JT  - Genes & development
JID - 8711660
RN  - 0 (Antigens, Polyomavirus Transforming)
RN  - 0 (Proto-Oncogene Proteins)
RN  - EC 2.7.10.2 (Proto-Oncogene Proteins c-yes)
RN  - EC 2.7.10.2 (Proto-Oncogene Proteins pp60(c-src))
RN  - EC 2.7.10.2 (Yes1 protein, mouse)
RN  - EC 2.7.10.2 (src-Family Kinases)
SB  - IM
MH  - Animals
MH  - Antigens, Polyomavirus Transforming/genetics
MH  - Enzyme Activation
MH  - Mammary Neoplasms, Experimental/*etiology/genetics
MH  - Mice
MH  - Mice, Transgenic
MH  - Oncogenes
MH  - Proto-Oncogene Proteins/physiology
MH  - Proto-Oncogene Proteins c-yes
MH  - Proto-Oncogene Proteins pp60(c-src)/*metabolism/physiology
MH  - *src-Family Kinases
EDAT- 1994/01/01 00:00
MHDA- 1994/01/01 00:01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1994/01/01 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
AID - 10.1101/gad.8.1.23 [doi]
PST - ppublish
SO  - Genes Dev. 1994 Jan;8(1):23-32. doi: 10.1101/gad.8.1.23.