PMID- 7507141
OWN - NLM
STAT- MEDLINE
DCOM- 19940222
LR  - 20170214
IS  - 0022-1554 (Print)
IS  - 0022-1554 (Linking)
VI  - 42
IP  - 2
DP  - 1994 Feb
TI  - Demonstration of acidity in intestinal vacuoles of the suckling rat and pig.
PG  - 231-8
AB  - Fluorescence staining characteristics of "large vacuoles," i.e. vacuoles ranging 
      up to almost cell size, were studied in suckling rats and pigs. In the distal 
      epithelium of the small intestine of suckling rat, yellow autofluorescence and 
      accumulation of orally administered FITC-dextran were observed in the 
      supranuclear vacuole. In both species the weakly basic amino dye acridine orange 
      (AO) stained the nuclei at neutral pH bright yellow-green and the transport and 
      digestive vacuoles bright red or orange. It is concluded that trapping and 
      accumulation of the dye (red shift) were due to the acidity of the vacuolar 
      interior. Assessment of the vacuolar pH in rat enterocytes is in agreement with 
      published data on lysosomal pH values. Acidic buffers, lysosomotropic and 
      destructive agents, or illumination with bright light induced irreversible fading 
      of AO-stained vacuoles; the color of the porcine transport vacuoles was the most 
      labile. This fading was used to differentiate vacuoles from other structures, 
      e.g., vacuolar inclusion bodies and goblet cells. In suckling rat, staining 
      characteristics of the gut epithelium changed on Days 19 and 20 of postnatal age. 
      Detection of acidity in the distal (digestive) vacuoles supports the 
      lysosome-like nature of their function. They appear to constitute an auxiliary, 
      intracellular digestive system for the young animal. However, the function of 
      acidity in the non-digestive transport vacuoles of newborn pig is unclear.
FAU - Baintner, K
AU  - Baintner K
AD  - Department of Physiology, Faculty of Animal Science, Pannon Agricultural 
      University, Kaposvar, Hungary.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Histochem Cytochem
JT  - The journal of histochemistry and cytochemistry : official journal of the 
      Histochemistry Society
JID - 9815334
RN  - 0 (Acids)
RN  - 0 (Dextrans)
RN  - 0 (fluorescein isothiocyanate dextran)
RN  - F30N4O6XVV (Acridine Orange)
RN  - I223NX31W9 (Fluorescein-5-isothiocyanate)
SB  - IM
MH  - Acids
MH  - Acridine Orange
MH  - Animals
MH  - Animals, Suckling/*physiology
MH  - Dextrans
MH  - Epithelial Cells
MH  - Epithelium/metabolism
MH  - Female
MH  - Fluorescein-5-isothiocyanate/analogs & derivatives
MH  - Hydrogen-Ion Concentration
MH  - Intestinal Mucosa/cytology/*metabolism
MH  - Intestine, Small/cytology/physiology
MH  - Lysosomes/metabolism
MH  - Microscopy, Fluorescence
MH  - Rats
MH  - Rats, Wistar
MH  - Swine
MH  - Vacuoles/*metabolism
EDAT- 1994/02/01 00:00
MHDA- 1994/02/01 00:01
CRDT- 1994/02/01 00:00
PHST- 1994/02/01 00:00 [pubmed]
PHST- 1994/02/01 00:01 [medline]
PHST- 1994/02/01 00:00 [entrez]
AID - 10.1177/42.2.7507141 [doi]
PST - ppublish
SO  - J Histochem Cytochem. 1994 Feb;42(2):231-8. doi: 10.1177/42.2.7507141.