PMID- 7507695
OWN - NLM
STAT- MEDLINE
DCOM- 19940310
LR  - 20191023
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 9
IP  - 1
DP  - 1994 Jan
TI  - Cytogenetic and molecular analysis of 6q deletions in Burkitt's lymphoma cell 
      lines.
PG  - 13-8
AB  - Although abnormalities of chromosome 6 have frequently been observed in Burkitt's 
      lymphoma (BL), they have so far not been defined by modern cytogenetic and 
      molecular methods. By a combination of high-resolution chromosome banding, 
      fluorescence in situ hybridization (FISH), and loss of heterozygosity (LOH) 
      analysis, we have examined the nature of aberrations affecting chromosome 6 in 7 
      previously established BL cell lines. All cell lines exhibited the characteristic 
      translocations associated with BL; 5 had t(8;14)(q24;q32) and 2 had 
      t(8;22)(q24;q11). Three cell lines had deletions of 6q; 3 others had 
      rearrangements affecting 6q, whereas one cell line had apparently normal 
      chromosomes 6. FISH analysis of the three deletions established that they were 
      interstitial. LOH analysis with probes mapped to the 6q26-27 region confirmed the 
      sub-telomeric interstitial deletion in cell line BL-108, which had a 
      del(6)(q23q27). All informative loci mapped to 6q26-27 (5/7) were deleted in 
      BL-74, which had no apparent cytogenetic abnormality in chromosome 6, thus 
      documenting a sub-microscopic deletion. These data define the cytogenetic and 
      molecular limits of 6q deletions in BL and are consistent with our previous 
      demonstration of LOH analysis of the site of a candidate tumor suppressor gene in 
      the 6q25-27 region.
FAU - Parsa, N Z
AU  - Parsa NZ
AD  - Cell Biology and Genetics Program, Memorial Sloan-Kettering Cancer Center, New 
      York, NY 10021.
FAU - Gaidano, G
AU  - Gaidano G
FAU - Mukherjee, A B
AU  - Mukherjee AB
FAU - Hauptschein, R S
AU  - Hauptschein RS
FAU - Lenoir, G
AU  - Lenoir G
FAU - Dalla-Favera, R
AU  - Dalla-Favera R
FAU - Chaganti, R S
AU  - Chaganti RS
LA  - eng
GR  - CA-08748/CA/NCI NIH HHS/United States
GR  - CA-34775/CA/NCI NIH HHS/United States
GR  - CA-44029/CA/NCI NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Adolescent
MH  - Burkitt Lymphoma/*genetics
MH  - Child
MH  - Chromosome Banding
MH  - *Chromosome Deletion
MH  - *Chromosomes, Human, Pair 14
MH  - *Chromosomes, Human, Pair 22
MH  - *Chromosomes, Human, Pair 6
MH  - DNA, Neoplasm/analysis
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Tumor Cells, Cultured
EDAT- 1994/01/01 00:00
MHDA- 1994/01/01 00:01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1994/01/01 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
AID - 10.1002/gcc.2870090104 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 1994 Jan;9(1):13-8. doi: 10.1002/gcc.2870090104.