PMID- 7508754
OWN - NLM
STAT- MEDLINE
DCOM- 19940321
LR  - 20191210
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1220
IP  - 2
DP  - 1994 Jan 13
TI  - Effect of inhibition of microsomal Ca(2+)-ATPase on cytoplasmic calcium and 
      enzyme secretion in pancreatic acini.
PG  - 199-208
AB  - We used thapsigargin (TG), 2,5-di-tert-butyl-1,4-benzohydroquinone (BHQ) and 
      cyclopiazonic acid (CPA), each of which inhibits microsomal Ca(2+)-ATPase, to 
      evaluate the effects of this inhibition on cytoplasmic free calcium ([Ca2+]i) and 
      secretagogue-stimulated enzyme secretion in rat pancreatic acini. Using 
      single-cell microspectrofluorimetry of fura-2-loaded acini we found that all 
      three agents caused a sustained increase in [Ca2+]i by mobilizing calcium from 
      inositol-(1,4,5)-trisphosphate-sensitive intracellular calcium stores and by 
      promoting influx of extracellular calcium. Concentrations of all three agents 
      that increased [Ca2+]i potentiated the stimulation of enzyme secretion caused by 
      secretagogues that activate adenylate cyclase but inhibited the stimulation of 
      enzyme secretion caused by secretagogues that activate phospholipase C. With BHQ, 
      potentiation of adenylate cyclase-mediated enzyme secretion occurred immediately 
      whereas inhibition of phospholipase C-mediated enzyme secretion occurred only 
      after several min of incubation. In addition, the effects of BHQ and CPA on both 
      [Ca2+]i and secretagogue-stimulated enzyme secretion were reversed completely by 
      washing whereas the actions of TG could not be reversed by washing. 
      Concentrations of BHQ in excess of those that caused maximal changes in [Ca2+]i 
      inhibited all modes of stimulated enzyme secretion by a mechanism that was 
      apparently unrelated to changes in [Ca2+]i. Finally, in contrast to the findings 
      with TG and BHQ, CPA inhibited bombesin-stimulated enzyme secretion over a range 
      of concentrations that was at least 10-fold lower than the range of 
      concentrations over which CPA potentiated VIP-stimulated enzyme secretion.
FAU - Metz, D C
AU  - Metz DC
AD  - Digestive Disease Branch, National Institute of Diabetes and Digestive and Kidney 
      Diseases, National Institutes of Health, Bethesda, MD 20892.
FAU - Pradhan, T K
AU  - Pradhan TK
FAU - Mrozinski, J E Jr
AU  - Mrozinski JE Jr
FAU - Jensen, R T
AU  - Jensen RT
FAU - Turner, R J
AU  - Turner RJ
FAU - Patto, R J
AU  - Patto RJ
FAU - Gardner, J D
AU  - Gardner JD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Hydroquinones)
RN  - 0 (Indoles)
RN  - 26XK13B61B (2,5-di-tert-butylhydroquinone)
RN  - EC 3.2.1.- (Amylases)
RN  - EC 7.2.2.10 (Calcium-Transporting ATPases)
RN  - M03GIQ7Z6P (Sincalide)
RN  - SY7Q814VUP (Calcium)
RN  - X9TLY4580Z (cyclopiazonic acid)
SB  - IM
MH  - Amylases/*metabolism
MH  - Animals
MH  - Calcium/*metabolism
MH  - Calcium-Transporting ATPases/*antagonists & inhibitors
MH  - Cytoplasm/metabolism
MH  - Hydroquinones/pharmacology
MH  - Indoles/pharmacology
MH  - Male
MH  - Microsomes/enzymology
MH  - Pancreas/*metabolism/ultrastructure
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sincalide/pharmacology
EDAT- 1994/01/13 00:00
MHDA- 2001/03/28 10:01
CRDT- 1994/01/13 00:00
PHST- 1994/01/13 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1994/01/13 00:00 [entrez]
AID - 0167-4889(94)90136-8 [pii]
AID - 10.1016/0167-4889(94)90136-8 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1994 Jan 13;1220(2):199-208. doi: 
      10.1016/0167-4889(94)90136-8.