PMID- 7509197
OWN - NLM
STAT- MEDLINE
DCOM- 19940325
LR  - 20151119
IS  - 0792-8483 (Print)
IS  - 0792-8483 (Linking)
VI  - 4
IP  - 1
DP  - 1993 Jan-Mar
TI  - The distribution of astrocytes, oligodendroglia and myelin in normal and 
      transplanted rat superior colliculus: an immunohistochemical study.
PG  - 1-14
AB  - Immunohistochemical methods have been used to determine the distribution of 
      macroglia and myelin in the normal rat superior colliculus (SC) and in grafts of 
      fetal tectal tissue. The fetal tissue was derived from 15 day-old (E15) rat 
      embryos and was transplanted onto the midbrain of newborn host rats of the same 
      (PVG/c) strain. Antibodies to glial fibrillary acidic protein (GFAP) and carbonic 
      anhydrase II (CAII) were used to visualize astrocytes and oligodendroglia 
      respectively. Myelin was immunostained with antibodies to either proteolipid 
      protein (PLP) or myelin basic protein (MBP). In the normal SC, GFAP positive 
      astrocytes were found scattered throughout the SC, particularly in the 
      superficial layers. They were especially prominent at the pial surface, around 
      major blood vessels and at the midline between the two colliculi. CAII 
      immunoreactive oligodendroglia and associated myelin were also found throughout 
      the SC; by far the lowest density was seen in the stratum griseum superficiale 
      (SGS). Both types of macroglia cell were found in abundance in tectal 
      transplants, indicating that the precursors of these glial types were present in 
      the E15 rat mesencephalon. In mature grafts, large numbers of fibrous astrocytes 
      were found throughout the neuropil and the level of GFAP immunoreactivity was 
      consistently greater than in host SC. Astrocytes seemed to be maintained in a 
      reactive, perhaps immature state within the grafted tissue. Tectal transplants 
      possessed large numbers of fully differentiated CAII-positive oligodendroglia and 
      the grafts contained a dense network of myelinated axons. However the 
      distribution of CAII and PLP immunoreactivity was not homogeneous; there were 
      localized, well-defined regions that contained few oligodendroglia and relatively 
      little myelin. These areas stained intensely for acetylcholinesterase (AChE) and 
      were almost certainly homologous to the SGS of normal SC. The relative lack of 
      oligodendroglia in the AChE stained patches in grafts and in SGS in situ suggests 
      that local factors influencing the proliferation and distribution of 
      oligodendroglia in normal SC may have been operating in a similar manner within 
      the tectal transplant neuropil.
FAU - Harvey, A R
AU  - Harvey AR
AD  - Department of Anatomy and Human Biology, University of Western Australia, 
      Nedlands, Perth.
FAU - Plant, G W
AU  - Plant GW
FAU - Kent, A P
AU  - Kent AP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Neural Transplant Plast
JT  - Journal of neural transplantation & plasticity
JID - 9104161
RN  - 0 (Biomarkers)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Myelin Basic Protein)
RN  - 0 (Myelin Proteins)
RN  - 0 (Myelin Proteolipid Protein)
RN  - 0 (Nerve Tissue Proteins)
RN  - EC 3.1.1.7 (Acetylcholinesterase)
RN  - EC 4.2.1.1 (Carbonic Anhydrases)
SB  - IM
MH  - Acetylcholinesterase/analysis
MH  - Animals
MH  - *Astrocytes/chemistry
MH  - Biomarkers/analysis
MH  - Brain Tissue Transplantation/*pathology
MH  - Carbonic Anhydrases/analysis
MH  - Fetal Tissue Transplantation/*pathology
MH  - Fluorescent Antibody Technique
MH  - Glial Fibrillary Acidic Protein/analysis
MH  - Mesencephalon/pathology
MH  - Myelin Basic Protein/analysis
MH  - Myelin Proteins/analysis
MH  - Myelin Proteolipid Protein
MH  - *Myelin Sheath
MH  - Nerve Tissue Proteins/*analysis
MH  - *Oligodendroglia/chemistry
MH  - Rats
MH  - Superior Colliculi/*cytology/embryology/*transplantation
PMC - PMC2565246
EDAT- 1993/01/01 00:00
MHDA- 1993/01/01 00:01
PMCR- 1993/01/01
CRDT- 1993/01/01 00:00
PHST- 1993/01/01 00:00 [pubmed]
PHST- 1993/01/01 00:01 [medline]
PHST- 1993/01/01 00:00 [entrez]
PHST- 1993/01/01 00:00 [pmc-release]
AID - 10.1155/NP.1993.1 [doi]
PST - ppublish
SO  - J Neural Transplant Plast. 1993 Jan-Mar;4(1):1-14. doi: 10.1155/NP.1993.1.