PMID- 7510778
OWN - NLM
STAT- MEDLINE
DCOM- 19940420
LR  - 20190630
IS  - 0022-3042 (Print)
IS  - 0022-3042 (Linking)
VI  - 62
IP  - 4
DP  - 1994 Apr
TI  - Tumor necrosis factor-alpha (TNF-alpha), interferon-gamma, and interleukin-6 but 
      not TNF-beta induce differentiation of neuroblastoma cells: the role of nitric 
      oxide.
PG  - 1330-6
AB  - Tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma), and 
      interleukin-6 (IL-6), but not TNF-beta, can induce the in vitro differentiation 
      of the neuroblastoma cell line N103 in a dose-dependent manner. Differentiation 
      of N103 was accompanied by the arrest of cell growth and neurite formation. The 
      induction of neuroblastoma cell differentiation by TNF-alpha and IFN-gamma can be 
      specifically inhibited by a nitric oxide (NO) synthase inhibitor, 
      L-NG-monomethylarginine. In contrast, the differentiation of N103 cells by IL-6 
      was not affected by L-NG-monomethylarginine. These results indicate that 
      TNF-alpha and IFN-gamma, but not IL-6, induce the differentiation of 
      neuroblastoma cells via NO. This is confirmed by the finding that the culture 
      supernatants of N103 cells induced by TNF-alpha and IFN-gamma, but not that by 
      IL-6, contained high levels of NO2-, the production of which was inhibited by 
      L-NG-monomethylarginine. Furthermore, the differentiation of N103 cells can be 
      induced directly in a dose-dependent manner by the addition of nitroprusside, a 
      generator of NO, into the culture medium. These data therefore indicate that NO 
      may be an important mediator in the induction of neuronal cell differentiation by 
      certain cytokines such as TNF-alpha and IFN-gamma and that neuronal cells, in 
      addition to the macrophage-like brain cells, can be induced by immunological 
      stimuli to produce large quantities of NO.
FAU - Munoz-Fernandez, M A
AU  - Munoz-Fernandez MA
AD  - Centro de Biologia Molecular Severo Ochoa (CSIC-UAM), Universidad Autonoma de 
      Madrid, Spain.
FAU - Cano, E
AU  - Cano E
FAU - O'Donnell, C A
AU  - O'Donnell CA
FAU - Doyle, J
AU  - Doyle J
FAU - Liew, F Y
AU  - Liew FY
FAU - Fresno, M
AU  - Fresno M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (Interleukin-6)
RN  - 0 (Lymphotoxin-alpha)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 27JT06E6GR (omega-N-Methylarginine)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 9007-49-2 (DNA)
RN  - 94ZLA3W45F (Arginine)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.4.- (Amino Acid Oxidoreductases)
SB  - IM
MH  - Amino Acid Oxidoreductases/antagonists & inhibitors
MH  - Animals
MH  - Arginine/analogs & derivatives/pharmacology
MH  - *Cell Differentiation
MH  - DNA/biosynthesis
MH  - Interferon-gamma/*pharmacology
MH  - Interleukin-6/*pharmacology
MH  - Lymphotoxin-alpha/pharmacology
MH  - Mice
MH  - Neuroblastoma/*pathology
MH  - Nitric Oxide/*physiology
MH  - Nitric Oxide Synthase
MH  - Recombinant Proteins/pharmacology
MH  - Tumor Cells, Cultured
MH  - Tumor Necrosis Factor-alpha/*pharmacology
MH  - omega-N-Methylarginine
EDAT- 1994/04/01 00:00
MHDA- 1994/04/01 00:01
CRDT- 1994/04/01 00:00
PHST- 1994/04/01 00:00 [pubmed]
PHST- 1994/04/01 00:01 [medline]
PHST- 1994/04/01 00:00 [entrez]
AID - 10.1046/j.1471-4159.1994.62041330.x [doi]
PST - ppublish
SO  - J Neurochem. 1994 Apr;62(4):1330-6. doi: 10.1046/j.1471-4159.1994.62041330.x.