PMID- 7510962
OWN - NLM
STAT- MEDLINE
DCOM- 19940421
LR  - 20061115
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 199
IP  - 2
DP  - 1994 Mar 15
TI  - Porcine luteal cells express monocyte chemoattractant protein-1 (MCP-1): analysis 
      by polymerase chain reaction and cDNA cloning.
PG  - 962-8
AB  - RT PCR employing poly(A+)RNA from porcine luteal cells and a combination of 
      primers designed from the known bovine MCP-1 cDNA identified the luteal cells as 
      a source of MCP-1. This finding is corroborated by results from Northern analysis 
      using total RNA from luteal cells. To characterize the complete porcine MCP-1 
      cDNA, poly (A+)RNA was isolated from porcine corpus luteum, transcribed into cDNA 
      and the latter cloned into the expression vector lambda Uni-ZapXR. A 
      digoxigenin-labeled DNA probe of 375 bp was obtained by PCR and employed to 
      screen the library. From the positive clones pMCP5, pMCP7 and pMCP10, the clone 
      pMCP5 was selected and both strands of the cDNA insert were sequenced. The cDNA 
      insert was 742 bp long, with an open reading frame (ORF) encoding a protein of 99 
      amino acid residues which by comparison with known amino acid sequences of MCPs 
      yielded highest identities with MCP-1 sequences. We therefore assume that pMCP5 
      encodes the amino acid sequence for porcine MCP-1.
FAU - Hosang, K
AU  - Hosang K
AD  - Max-Planck-Institut fur Biophysikalische Chemie, Abt. Molekulare Biologie, 
      Gottingen, Germany.
FAU - Knoke, I
AU  - Knoke I
FAU - Klaudiny, J
AU  - Klaudiny J
FAU - Wempe, F
AU  - Wempe F
FAU - Wuttke, W
AU  - Wuttke W
FAU - Scheit, K H
AU  - Scheit KH
LA  - eng
SI  - GENBANK/Z48479
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemotactic Factors)
RN  - 0 (Cytokines)
RN  - 0 (DNA Primers)
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Messenger)
RN  - 24937-83-5 (Poly A)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cattle
MH  - Chemokine CCL2
MH  - Chemotactic Factors/*biosynthesis
MH  - Cloning, Molecular/methods
MH  - Corpus Luteum/*metabolism
MH  - Cytokines/*biosynthesis
MH  - DNA Primers
MH  - DNA Probes
MH  - DNA, Complementary/metabolism
MH  - Female
MH  - Humans
MH  - Molecular Sequence Data
MH  - Open Reading Frames
MH  - Poly A/isolation & purification/metabolism
MH  - Polymerase Chain Reaction/methods
MH  - RNA/isolation & purification/metabolism
MH  - RNA, Messenger
MH  - Sequence Homology, Amino Acid
MH  - Swine
EDAT- 1994/03/15 00:00
MHDA- 1994/03/15 00:01
CRDT- 1994/03/15 00:00
PHST- 1994/03/15 00:00 [pubmed]
PHST- 1994/03/15 00:01 [medline]
PHST- 1994/03/15 00:00 [entrez]
AID - S0006-291X(84)71323-4 [pii]
AID - 10.1006/bbrc.1994.1323 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1994 Mar 15;199(2):962-8. doi: 
      10.1006/bbrc.1994.1323.