PMID- 7511290
OWN - NLM
STAT- MEDLINE
DCOM- 19940425
LR  - 20061115
IS  - 0070-4113 (Print)
IS  - 0070-4113 (Linking)
VI  - 77
DP  - 1993
TI  - [Demonstration of gene amplification in urinary bladder cancer by fluorescent in 
      situ hybridization (FISH)].
PG  - 247-51
AB  - Fluorescence in situ hybridization (FISH) allows visualization of chromosomes and 
      genes in interphase nuclei. Dual labeling FISH with probes for a gene of interest 
      and the corresponding centromere can be used to determine chromosomal deletion 
      and gene amplification. In case of a deletion less gene signals than centromere 
      signals are found. In case of amplification gene signal number is distinctly 
      increased as compared to centromere signals. To study gene amplification in fresh 
      and formalin fixed bladder cancer we used gene specific probes for erbB-2, EGF-r, 
      and 11q13 (bcl-1, PRAD1) together with their corresponding centromere probes 
      p17H8, p7alphaTET and plC11A. Amplification was seen in 10/140 tumors for erbB-2, 
      in 5/107 tumors for EGF-r, and in 15/137 tumors for 11q13. Different patterns of 
      amplification suggested that FISH allows distinction of intrachromosomal 
      (amplified genes in clusters) and extrachromosomal amplification (diffuse 
      distribution of signals).
FAU - Sauter, G
AU  - Sauter G
AD  - Institut fur Pathologie, Universitat Basel.
FAU - Moch, H
AU  - Moch H
FAU - Gudat, F
AU  - Gudat F
FAU - Mihatsch, M J
AU  - Mihatsch MJ
FAU - Haley, J
AU  - Haley J
FAU - Meecker, T
AU  - Meecker T
FAU - Waldman, F
AU  - Waldman F
LA  - ger
PT  - English Abstract
PT  - Journal Article
TT  - Nachweis von Genamplifikation in Harnblasenkarzinomen mittels Fluoreszenz in situ 
      Hybridisierung (FISH).
PL  - Germany
TA  - Verh Dtsch Ges Pathol
JT  - Verhandlungen der Deutschen Gesellschaft fur Pathologie
JID - 7503704
SB  - IM
GS  - EGF-r
GS  - bcl-1
GS  - erbB-2
MH  - Carcinoma, Transitional Cell/*genetics/*pathology
MH  - Chromosomes, Human, Pair 11
MH  - Chromosomes, Human, Pair 17
MH  - Chromosomes, Human, Pair 7
MH  - *Gene Amplification
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Oncogenes
MH  - Urinary Bladder Neoplasms/*genetics/*pathology
EDAT- 1993/01/01 00:00
MHDA- 1993/01/01 00:01
CRDT- 1993/01/01 00:00
PHST- 1993/01/01 00:00 [pubmed]
PHST- 1993/01/01 00:01 [medline]
PHST- 1993/01/01 00:00 [entrez]
PST - ppublish
SO  - Verh Dtsch Ges Pathol. 1993;77:247-51.