PMID- 7511869
OWN - NLM
STAT- MEDLINE
DCOM- 19940505
LR  - 20190825
IS  - 0065-4299 (Print)
IS  - 0065-4299 (Linking)
VI  - 40
IP  - 1-2
DP  - 1993 Sep
TI  - Some characteristics of the ATP-induced histamine release from and 
      permeabilization of rat mast cells.
PG  - 18-27
AB  - Extracellular adenosine 5'-triphosphate (ATP) induced a characteristic, 
      dose-dependent release of histamine and prostaglandin D2 (PGD2) from rat 
      peritoneal mast cells. The process was relatively slow, non-cytotoxic, maximal at 
      physiological pH and dependent on external calcium. Strontium and barium ions 
      were able to substitute for calcium, although higher concentrations were required 
      for maximal release. Cells stimulated in the absence of calcium progressively 
      lost the ability to respond to subsequent reintroduction of the cation. The 
      secretion of histamine induced by ATP was largely unaffected by the 
      anti-asthmatic drugs disodium cromoglycate and nedocromil sodium but was 
      inhibited by structurally related flavonoids and by cAMP-active drugs. 
      Importantly, the non-hydrolysable guanosine 5'-triphosphate (GTP) analogue, 
      GTP-gamma-S, elicited a dose-dependent release of histamine when introduced into 
      mast cells permeabilized with ATP in the absence of external calcium. ATP thus 
      appears to be a useful cell permeabilizing tool with which to study the 
      biochemical processes involved in mast cell activation.
FAU - Jaffar, Z H
AU  - Jaffar ZH
AD  - Department of Chemistry, University College London, UK.
FAU - Pearce, F L
AU  - Pearce FL
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Agents Actions
JT  - Agents and actions
JID - 0213341
RN  - 0 (Chromones)
RN  - 0 (Metals)
RN  - 37589-80-3 (Guanosine 5'-O-(3-Thiotriphosphate))
RN  - 85166-31-0 (Inositol 1,4,5-Trisphosphate)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - E0399OZS9N (Cyclic AMP)
RN  - RXY07S6CZ2 (Prostaglandin D2)
SB  - IM
MH  - Adenosine Triphosphate/*pharmacology
MH  - Animals
MH  - Cell Membrane Permeability/*drug effects
MH  - Chromones/pharmacology
MH  - Cyclic AMP/metabolism
MH  - Guanosine 5'-O-(3-Thiotriphosphate)/pharmacology
MH  - Histamine Release/*drug effects
MH  - In Vitro Techniques
MH  - Inositol 1,4,5-Trisphosphate/pharmacology
MH  - Mast Cells/drug effects/*metabolism
MH  - Metals/pharmacology
MH  - Molecular Weight
MH  - Peritoneal Cavity/cytology
MH  - Prostaglandin D2/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 1993/09/01 00:00
MHDA- 1993/09/01 00:01
CRDT- 1993/09/01 00:00
PHST- 1993/09/01 00:00 [pubmed]
PHST- 1993/09/01 00:01 [medline]
PHST- 1993/09/01 00:00 [entrez]
AID - 10.1007/BF01976747 [doi]
PST - ppublish
SO  - Agents Actions. 1993 Sep;40(1-2):18-27. doi: 10.1007/BF01976747.