PMID- 7513803
OWN - NLM
STAT- MEDLINE
DCOM- 19940609
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 307
IP  - 1
DP  - 1994 May 1
TI  - Isolation of micronuclei from mouse blood and fluorescence in situ hybridization 
      with a mouse centromeric DNA probe.
PG  - 245-51
AB  - Spontaneously existing and chemically induced micronuclei were isolated from 
      mouse blood. 50 microliters of cardiac blood was diluted with PBS and 
      centrifuged. After this, the cell pellet was subjected to hypotonic treatment, 
      fixed with acetic acid-methanol (1:3), and the lysate was filtrated through a 
      2-microns polycarbonate nucleopore membrane. Isolated micronuclei were air-dried 
      on a glass slide and subjected to fluorescence in situ hybridization (FISH) using 
      a mouse centromeric gamma satellite probe. Approximately half of the micronuclei 
      isolated from vehicle control mice showed centromere signal(s). In these 
      preliminary studies, the proportion of centromere-positive micronuclei was 
      increased by treatment with spindle poisons (colchicine and vinblastine sulfate), 
      decreased only slightly by 1-beta-D-arabinofuranosylcytosine, and was generally 
      unaffected by mitomycin C.
FAU - Hayashi, M
AU  - Hayashi M
AD  - Division of Genetics and Mutagenesis, National Institute of Health Sciences, 
      Tokyo, Japan.
FAU - Maki-Paakkanen, J
AU  - Maki-Paakkanen J
FAU - Tanabe, H
AU  - Tanabe H
FAU - Honma, M
AU  - Honma M
FAU - Suzuki, T
AU  - Suzuki T
FAU - Matsuoka, A
AU  - Matsuoka A
FAU - Mizusawa, H
AU  - Mizusawa H
FAU - Sofuni, T
AU  - Sofuni T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (DNA Probes)
RN  - 04079A1RDZ (Cytarabine)
RN  - 50SG953SK6 (Mitomycin)
RN  - 5V9KLZ54CY (Vinblastine)
RN  - 9007-49-2 (DNA)
RN  - SML2Y3J35T (Colchicine)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cell Fractionation/*methods
MH  - *Centromere
MH  - Colchicine/toxicity
MH  - Cytarabine/toxicity
MH  - DNA
MH  - *DNA Probes
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Mice
MH  - Micronucleus Tests/*methods
MH  - Mitomycin/toxicity
MH  - Molecular Sequence Data
MH  - Vinblastine/toxicity
EDAT- 1994/05/01 00:00
MHDA- 1994/05/01 00:01
CRDT- 1994/05/01 00:00
PHST- 1994/05/01 00:00 [pubmed]
PHST- 1994/05/01 00:01 [medline]
PHST- 1994/05/01 00:00 [entrez]
AID - 0027-5107(94)90298-4 [pii]
AID - 10.1016/0027-5107(94)90298-4 [doi]
PST - ppublish
SO  - Mutat Res. 1994 May 1;307(1):245-51. doi: 10.1016/0027-5107(94)90298-4.