PMID- 7515058
OWN - NLM
STAT- MEDLINE
DCOM- 19940630
LR  - 20210210
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 269
IP  - 22
DP  - 1994 Jun 3
TI  - Suppression of antioxidative enzyme expression by transforming growth factor-beta 
      1 in rat hepatocytes.
PG  - 15488-92
AB  - We have investigated the effect of transforming growth factor-beta 1 (TGF-beta 1) 
      and three cytokines on expression of antioxidative enzymes, manganese-superoxide 
      dismutase, copper, zinc-superoxide dismutase, and catalase in cultured 
      hepatocytes of rat. While interleukin-1 beta and interleukin-6 induced 
      manganese-superoxide dismutase gene expression, they slightly suppressed catalase 
      gene expression in rat hepatocytes. TGF-beta 1 suppressed expression of all these 
      antioxidative enzymes in time- and cell density-dependent manners. Furthermore, 
      we examined the effect of TGF-beta 1 on expression of glutathione peroxidase and 
      glutathione-S-transferase, which exhibit glutathione-dependent peroxidase 
      activity in rat hepatocytes. Expression of two major classes of the rat 
      glutathione-S-transferase subunits 1 and 2 was also reduced by TGF-beta 1, 
      although expression of glutathione peroxidase was not affected. Flow cytometric 
      analysis indicated that production of peroxides was increased in hepatocytes 
      treated with TGF-beta 1. These data suggest that augmented production of hydrogen 
      peroxide and its intermediate through suppression of antioxidative enzyme 
      expression may participate in cellular injury or growth inhibition promoted by 
      TGF-beta 1.
FAU - Kayanoki, Y
AU  - Kayanoki Y
AD  - Department of Biochemistry, Osaka University Medical School, Japan.
FAU - Fujii, J
AU  - Fujii J
FAU - Suzuki, K
AU  - Suzuki K
FAU - Kawata, S
AU  - Kawata S
FAU - Matsuzawa, Y
AU  - Matsuzawa Y
FAU - Taniguchi, N
AU  - Taniguchi N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-6)
RN  - 0 (Isoenzymes)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor beta)
RN  - 63231-63-0 (RNA)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 2.5.1.18 (Glutathione Transferase)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - Catalase/*biosynthesis
MH  - Cells, Cultured
MH  - Cytokines/pharmacology
MH  - Enzyme Induction
MH  - Gene Expression/drug effects
MH  - Glutathione Peroxidase/*biosynthesis
MH  - Glutathione Transferase/biosynthesis
MH  - Interleukin-1/pharmacology
MH  - Interleukin-6/pharmacology
MH  - Isoenzymes/biosynthesis
MH  - Liver/drug effects/*enzymology
MH  - Male
MH  - RNA/biosynthesis/isolation & purification
MH  - RNA, Messenger/analysis/*biosynthesis/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Superoxide Dismutase/*biosynthesis
MH  - Transforming Growth Factor beta/*pharmacology
EDAT- 1994/06/03 00:00
MHDA- 1994/06/03 00:01
CRDT- 1994/06/03 00:00
PHST- 1994/06/03 00:00 [pubmed]
PHST- 1994/06/03 00:01 [medline]
PHST- 1994/06/03 00:00 [entrez]
AID - S0021-9258(17)40705-8 [pii]
PST - ppublish
SO  - J Biol Chem. 1994 Jun 3;269(22):15488-92.