PMID- 7515284
OWN - NLM
STAT- MEDLINE
DCOM- 19940705
LR  - 20190512
IS  - 0006-3363 (Print)
IS  - 0006-3363 (Linking)
VI  - 50
IP  - 4
DP  - 1994 Apr
TI  - A growth factor phenotype map for ovine preimplantation development.
PG  - 725-33
AB  - The reverse transcription-polymerase chain reaction (RT-PCR) was used to 
      determine the patterns of expression for several growth factor ligand and 
      receptor genes during ovine preimplantation development. Transcripts for 
      insulin-like growth factor (IGF)-I, IGF-II, and the receptors for insulin and 
      IGF-I were detected throughout ovine preimplantation development from the 1-cell 
      to the blastocyst stage. Transforming growth factor alpha (TGF alpha) transcripts 
      were also detected throughout ovine preimplantation development. The mRNAs 
      encoding basic fibroblast growth factor (bFGF) were detected in all stages of the 
      ovine preimplantation embryo, although the relative abundance of this transcript 
      consistently decreased from the 1-cell to the blastocyst stage, suggesting that 
      it may represent a maternal transcript in early sheep embryos. Transcripts 
      encoding ovine trophoblast protein (oTP) were detected only within 
      blastocyst-stage embryos. Primary ovine oviduct cell cultures express the 
      transcripts for IGF-II, IGF-I, TGF alpha, bFGF, TGF beta 1, and the receptors for 
      insulin and IGF-I, suggesting that paracrine growth factor circuits may exist 
      between the oviduct epithelium and the early ovine embryo. Transcripts for 
      insulin, epidermal growth factor (EGF), and nerve growth factor (NGF) were not 
      detected in any stage of the ovine preimplantation embryo or within the oviduct 
      cell preparations. The expression of growth factor transcripts very early in 
      mammalian development would predict that these molecules fulfil a necessary 
      role(s) in supporting the progression of early embryos through the 
      preimplantation interval. Our future efforts will be directed to understanding 
      the nature of these putative regulatory pathways.
FAU - Watson, A J
AU  - Watson AJ
AD  - Department of Obstetrics and Gynaecology, University of Western Ontario, London, 
      Canada.
FAU - Watson, P H
AU  - Watson PH
FAU - Arcellana-Panlilio, M
AU  - Arcellana-Panlilio M
FAU - Warnes, D
AU  - Warnes D
FAU - Walker, S K
AU  - Walker SK
FAU - Schultz, G A
AU  - Schultz GA
FAU - Armstrong, D T
AU  - Armstrong DT
FAU - Seamark, R F
AU  - Seamark RF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (Growth Substances)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor alpha)
RN  - 0 (Transforming Growth Factor beta)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
RN  - 67763-97-7 (Insulin-Like Growth Factor II)
RN  - EC 2.7.7.49 (RNA-Directed DNA Polymerase)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Blastocyst/*physiology
MH  - Cells, Cultured
MH  - Embryonic Development/*physiology
MH  - Fallopian Tubes/cytology/metabolism
MH  - Female
MH  - Fibroblast Growth Factor 2/genetics
MH  - Growth Substances/*genetics
MH  - Insulin-Like Growth Factor I/genetics
MH  - Insulin-Like Growth Factor II/genetics
MH  - Molecular Sequence Data
MH  - *Phenotype
MH  - Polymerase Chain Reaction
MH  - Pregnancy
MH  - RNA, Messenger/analysis
MH  - RNA-Directed DNA Polymerase
MH  - Sheep
MH  - Transforming Growth Factor alpha/genetics
MH  - Transforming Growth Factor beta/genetics
EDAT- 1994/04/01 00:00
MHDA- 1994/04/01 00:01
CRDT- 1994/04/01 00:00
PHST- 1994/04/01 00:00 [pubmed]
PHST- 1994/04/01 00:01 [medline]
PHST- 1994/04/01 00:00 [entrez]
AID - 10.1095/biolreprod50.4.725 [doi]
PST - ppublish
SO  - Biol Reprod. 1994 Apr;50(4):725-33. doi: 10.1095/biolreprod50.4.725.