PMID- 7515326
OWN - NLM
STAT- MEDLINE
DCOM- 19940707
LR  - 20190620
IS  - 0008-543X (Print)
IS  - 0008-543X (Linking)
VI  - 73
IP  - 12
DP  - 1994 Jun 15
TI  - Epstein-Barr virus positivity in Hodgkin's disease does not correlate with an HLA 
      A2-negative phenotype.
PG  - 3059-63
AB  - BACKGROUND: Epstein-Barr virus- (EBV) related DNA and RNA can be found in tissues 
      involved with Hodgkin's disease, specifically in the Reed-Sternberg cells. These 
      cells also express the membrane antigens LMP1 and LMP 2A and 2B. Studies in 
      normal individuals indicate that cellular immunity against LMP2 was frequently 
      mediated through human leukocyte antigen (HLA) A2, whereas responses to LMP1 
      appeared to be relatively infrequent. Assuming that LMP2-positive Reed-Sternberg 
      cells would be sensitive to a CD8-positive cellular immune response, the 
      hypothesis can be made that EBV-positive Hodgkin's disease should be more common 
      in individuals not expressing HLA A2. To test this hypothesis, the authors have 
      studied the frequency of HLA A2 in EBV-positive versus EBV-negative patients with 
      Hodgkin's disease. METHODS: All 72 patients diagnosed with Hodgkin's disease in 
      Northern and Central Alberta, Canada, during 1990 and 1991 were studied. A 
      nonisotopic in situ hybridization method with an oligonucleotide probe specific 
      for EBER 1 and 2 was used. In addition, sections were stained for the EBV-latent 
      protein LMP1, HLA A2, and a monomorphic HLA class I determinant and beta 
      2-microglobulin. RESULTS: EBER-positive Reed-Sternberg cells were found in 26% of 
      the patients. The percentage of positive patients was 86% in mixed cellularity, 
      13% in nodular sclerosis, and 0% in lymphocyte predominance. The number of those 
      who were HLA-A2 positive was approximately 50% in the EBV-positive and -negative 
      patients. CONCLUSIONS: Therefore, no correlation between HLA A2 expression and 
      presence or absence of EBV in the R-S cells of Hodgkin's disease was identified.
FAU - Poppema, S
AU  - Poppema S
AD  - Department of Laboratory Medicine, Cross Cancer Institute, Edmonton, Alberta, 
      Canada.
FAU - Visser, L
AU  - Visser L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer
JT  - Cancer
JID - 0374236
RN  - 0 (DNA, Viral)
RN  - 0 (HLA-A2 Antigen)
RN  - 0 (Oligonucleotide Probes)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Base Sequence
MH  - DNA, Viral/analysis
MH  - Female
MH  - HLA-A2 Antigen/*analysis
MH  - Herpesvirus 4, Human/genetics/*isolation & purification
MH  - Hodgkin Disease/*immunology/*microbiology
MH  - Humans
MH  - Male
MH  - Molecular Sequence Data
MH  - Oligonucleotide Probes
MH  - Phenotype
MH  - RNA/analysis
EDAT- 1994/06/15 00:00
MHDA- 1994/06/15 00:01
CRDT- 1994/06/15 00:00
PHST- 1994/06/15 00:00 [pubmed]
PHST- 1994/06/15 00:01 [medline]
PHST- 1994/06/15 00:00 [entrez]
AID - 10.1002/1097-0142(19940615)73:12<3059::aid-cncr2820731226>3.0.co;2-q [doi]
PST - ppublish
SO  - Cancer. 1994 Jun 15;73(12):3059-63. doi: 
      10.1002/1097-0142(19940615)73:12<3059::aid-cncr2820731226>3.0.co;2-q.