PMID- 7515657
OWN - NLM
STAT- MEDLINE
DCOM- 19940712
LR  - 20191023
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 9
IP  - 3
DP  - 1994 Mar
TI  - Polysomy of chromosome 12 in 60 patients with non-Hodgkin's lymphoma assessed by 
      fluorescence in situ hybridization: differences between follicular and diffuse 
      large cell lymphoma.
PG  - 161-7
AB  - Sixty consecutive evaluable specimens from patients with non-Hodgkin's lymphoma 
      (NHL) were studied for the incidence of polysomy of chromosome 12 by fluorescence 
      in situ hybridization (FISH) with probes for the repetitive DNA sequence in the 
      centromeric region of chromosome 12. Thirty-six samples were from follicular 
      lymphomas (FLs), and twenty-four were from diffuse large cell lymphomas (DLCLs). 
      Fifty-two specimens (86%) were obtained by fine-needle aspiration of a diseased 
      node, seven (11.6%) were from involved bone marrows, and one specimen was from a 
      pleural effusion. Twelve of the thirty-six (33%) cases with FL had trisomy 12 in 
      3-41% of the cells (median, 10%) (normal controls had three signals in 1.4 +/- 
      0.7% of cells). Trisomy 12 was found in 62% of the patients who had had FL for 
      more than 5 years. Nine of the twenty-four cases (37%) with DLCL had more than 
      two copies of chromosome 12 in 4-92% of the cells (median, 78%), and all nine 
      cases were of B-cell phenotype. Unlike FL cells, some DLCL cells had 4-6 copies 
      of chromosome 12. In previously untreated patients, 54% of DLCLs and 26% of FLs 
      had subpopulations of cells containing more than two copies of chromosome 12 (P = 
      0.04). Only 2/7 cases of DLCL with polysomy 12 had rearrangement of the BCL2 
      gene, indicating that the majority of DLCL cases with polysomy 12 did not result 
      from histologic transformation of low grade follicular lymphomas. These data 
      demonstrate that FISH of interphase cells is a sensitive method for detecting 
      numerical abnormalities of chromosome 12 in NHL.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Younes, A
AU  - Younes A
AD  - Division of Medicine, University of Texas M.D. Anderson Cancer Center, Houston 
      77030.
FAU - Pugh, W
AU  - Pugh W
FAU - Goodacre, A
AU  - Goodacre A
FAU - Katz, R
AU  - Katz R
FAU - Rodriguez, M A
AU  - Rodriguez MA
FAU - Hill, D
AU  - Hill D
FAU - Cabanillas, F
AU  - Cabanillas F
FAU - Andreeff, M
AU  - Andreeff M
LA  - eng
GR  - CA-16672/CA/NCI NIH HHS/United States
GR  - CA-55164/CA/NCI NIH HHS/United States
GR  - CA-57639/CA/NCI NIH HHS/United States
GR  - etc.
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (DNA, Neoplasm)
SB  - IM
GS  - BCL2
MH  - *Aneuploidy
MH  - *Chromosomes, Human, Pair 12
MH  - DNA, Neoplasm/analysis
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphoma, Follicular/genetics
MH  - Lymphoma, Large B-Cell, Diffuse/genetics
MH  - Lymphoma, Non-Hodgkin/classification/*genetics/pathology
MH  - Oncogenes
MH  - Repetitive Sequences, Nucleic Acid
MH  - Trisomy
EDAT- 1994/03/01 00:00
MHDA- 1994/03/01 00:01
CRDT- 1994/03/01 00:00
PHST- 1994/03/01 00:00 [pubmed]
PHST- 1994/03/01 00:01 [medline]
PHST- 1994/03/01 00:00 [entrez]
AID - 10.1002/gcc.2870090303 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 1994 Mar;9(3):161-7. doi: 10.1002/gcc.2870090303.