PMID- 7515718
OWN - NLM
STAT- MEDLINE
DCOM- 19940712
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 83
IP  - 12
DP  - 1994 Jun 15
TI  - RANTES- and interleukin-8-induced responses in normal human eosinophils: effects 
      of priming with interleukin-5.
PG  - 3697-704
AB  - We report that responses of normal human eosinophils toward the chemokines RANTES 
      and interleukin-8 (IL-8) are modulated and upregulated by priming with IL-5. In a 
      modified Boyden chamber assay, we studied migratory responses toward the members 
      of the chemokine family RANTES, monocyte chemotactic protein-1 (MCP-1), and 
      macrophage inflammatory protein-1 alpha (MIP-1 alpha) (C-C subfamily), and IL-8, 
      platelet factor-4 (PF-4), and neutrophil-activating peptide-2 (NAP-2) (C-x-C 
      subfamily). These chemokines were also studied in terms of actin polymerization 
      and ([Ca2+]i)-mobilizing properties, intracellular signals that are thought to 
      play a role during migratory responses. We found that eosinophils showed 
      significant migratory responses toward RANTES and IL-8 at concentrations of 
      10(-9) to 10(-7) mol/L only after priming with IL-5 (10 pmol/L). At these 
      concentrations, PF-4, NAP-2, MCP-1, and MIP-1 alpha induced no significant 
      migratory responses after priming. Unprimed eosinophils only showed a significant 
      migratory response toward RANTES (10(-6) mol/L). Changes in [Ca2+]i were found 
      after addition of RANTES, MIP-1 alpha, and NAP-2 (10 nmol/L) to unprimed 
      eosinophils. RANTES (10(-9) to 10(-7) mol/L) significantly induced actin 
      polymerization both in primed and unprimed eosinophils, whereas IL-8 (10(-9) to 
      10(-8) mol/L) and MIP-1 alpha (10(-8) mol/L) only induced actin polymerization 
      after priming with IL-5. NAP-2, PF-4, and MCP-1 did not affect actin 
      polymerization. These findings are further evidence for the hypothesis that 
      cytokines like IL-5 and locally secreted chemokines like RANTES and IL-8 are both 
      at the basis of specific eosinophil influx into the allergic inflammatory locus.
FAU - Schweizer, R C
AU  - Schweizer RC
AD  - Department of Pulmonary Diseases, University Hospital Utrecht, The Netherlands.
FAU - Welmers, B A
AU  - Welmers BA
FAU - Raaijmakers, J A
AU  - Raaijmakers JA
FAU - Zanen, P
AU  - Zanen P
FAU - Lammers, J W
AU  - Lammers JW
FAU - Koenderman, L
AU  - Koenderman L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Actins)
RN  - 0 (Chemokine CCL5)
RN  - 0 (Interleukin-5)
RN  - 0 (Interleukin-8)
RN  - 0 (Lymphokines)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Actins/metabolism
MH  - Calcium/metabolism
MH  - Cell Movement/drug effects
MH  - Chemokine CCL5
MH  - Eosinophils/*drug effects/physiology
MH  - Humans
MH  - Interleukin-5/*pharmacology
MH  - Interleukin-8/*pharmacology
MH  - Lymphokines/*pharmacology
EDAT- 1994/06/15 00:00
MHDA- 1994/06/15 00:01
CRDT- 1994/06/15 00:00
PHST- 1994/06/15 00:00 [pubmed]
PHST- 1994/06/15 00:01 [medline]
PHST- 1994/06/15 00:00 [entrez]
AID - S0006-4971(20)78868-3 [pii]
PST - ppublish
SO  - Blood. 1994 Jun 15;83(12):3697-704.