PMID- 7516797
OWN - NLM
STAT- MEDLINE
DCOM- 19940725
LR  - 20191111
IS  - 1071-2690 (Print)
IS  - 1071-2690 (Linking)
VI  - 30A
IP  - 2
DP  - 1994 Feb
TI  - Effect of hepatocyte growth factor/scatter factor and other growth factors on 
      motility and morphology of non-tumorigenic and tumor cells.
PG  - 105-10
AB  - Using an automated cell analyzer system, the effect of hepatocyte growth 
      factor/scatter factor (HGF/SF), epidermal growth factor (EGF), basic fibroblast 
      growth factor (bFGF), endothelial acidic fibroblast growth factor (a-FGF), 
      platelet derived growth factor (PDGF), and recombinant human insulinlike growth 
      factor (IGF) on the motility and morphology of Madin-Darby canine kidney (MDCK), 
      rat hepatomas, C2, and H5-6 and murine mammary carcinoma (EMT-6) cells was 
      investigated. Treatment of MDCK cells with HGF/SF, bFGF, EGF, and a-FGF resulted 
      in an increase in average cell velocity and in the fraction of moving cells. 
      Cells treated with the PDGF and IGF did not show significant alterations in 
      velocity. MDCK cells treated with each growth factor were classified into groups 
      of "fast" and "slow" moving cells based on their average velocities, and the 
      average morphologic features of the two groups were quantitated. Fast-moving 
      cells had larger average area, circularity, and flatness as compared to 
      slow-moving cells. Factors that stimulated cell movement also induced alterations 
      in cell morphologic parameters including spreading, flatness, area, and 
      circularity. HGF/SF also scattered and stimulated motility of C2 and H5-6 
      hepatoma cells. In contrast to MDCK cells, there was no significant difference 
      between the morphology of the fast moving and slow moving C2 and H5-6 cells. 
      These studies suggest that growth factor cytokines have specific effects on 
      motility of normal and tumor cells.
FAU - Li, Y
AU  - Li Y
AD  - Department of Radiation Oncology, Long Island Jewish Medical Center, Albert 
      Einstein College of Medicine, New Hyde Park, New York 11042.
FAU - Bhargava, M M
AU  - Bhargava MM
FAU - Joseph, A
AU  - Joseph A
FAU - Jin, L
AU  - Jin L
FAU - Rosen, E M
AU  - Rosen EM
FAU - Goldberg, I D
AU  - Goldberg ID
LA  - eng
GR  - CA-50516/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Germany
TA  - In Vitro Cell Dev Biol Anim
JT  - In vitro cellular & developmental biology. Animal
JID - 9418515
RN  - 0 (Growth Substances)
RN  - 0 (Platelet-Derived Growth Factor)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - 104781-85-3 (Fibroblast Growth Factor 1)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cell Line
MH  - Cell Movement
MH  - Dogs
MH  - Epidermal Growth Factor/pharmacology
MH  - Fibroblast Growth Factor 1/pharmacology
MH  - Fibroblast Growth Factor 2/pharmacology
MH  - Growth Substances/*pharmacology
MH  - Hepatocyte Growth Factor/*pharmacology
MH  - Humans
MH  - Kidney
MH  - Liver Neoplasms, Experimental/pathology
MH  - Mammary Neoplasms, Experimental/pathology
MH  - Mice
MH  - Platelet-Derived Growth Factor/pharmacology
MH  - Rats
MH  - Tumor Cells, Cultured
EDAT- 1994/02/01 00:00
MHDA- 1994/02/01 00:01
CRDT- 1994/02/01 00:00
PHST- 1994/02/01 00:00 [pubmed]
PHST- 1994/02/01 00:01 [medline]
PHST- 1994/02/01 00:00 [entrez]
AID - 10.1007/BF02631401 [doi]
PST - ppublish
SO  - In Vitro Cell Dev Biol Anim. 1994 Feb;30A(2):105-10. doi: 10.1007/BF02631401.