PMID- 7517746
OWN - NLM
STAT- MEDLINE
DCOM- 19940811
LR  - 20190116
IS  - 1042-8194 (Print)
IS  - 1026-8022 (Linking)
VI  - 13
IP  - 1-2
DP  - 1994 Mar
TI  - The study of acute leukemia cells by means of acridine orange staining and flow 
      cytometry.
PG  - 61-73
AB  - The studies described here explored the staining of acute leukemia cells with 
      acridine orange (AO). The red fluorescence curve of AML specimens was usually 
      bimodal, suggesting the presence of subpopulations of cells which have different 
      RNA contents. In almost every AML specimen, small leukemic blast cells comprised 
      at least part of the "low RNA content" subpopulation. Residual granulocytes and 
      lymphocytes also contributed to this population. Frequently, the green 
      fluorescence, indicative of the binding of AO to DNA, was slightly less in these 
      cells than in the majority of cells present. There was no evidence however, that 
      the leukemia cells with these characteristics represented a G0 or kinetically 
      quiescent population of cells. In ALL specimens, the presence of multiple 
      cytogenetically distinct clones was easily detectable in AO stained specimens. 
      The red fluorescence curve of G0/G1 ALL cells was unimodal.
FAU - Preisler, H D
AU  - Preisler HD
AD  - Rush Cancer Institute, Chicago, Illinois.
FAU - Raza, A
AU  - Raza A
FAU - Gopal, V
AU  - Gopal V
FAU - Banavali, S D
AU  - Banavali SD
FAU - Bokhari, J
AU  - Bokhari J
FAU - Lampkin, B
AU  - Lampkin B
LA  - eng
GR  - CA41285/CA/NCI NIH HHS/United States
GR  - CA60085/CA/NCI NIH HHS/United States
GR  - CA60086/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Leuk Lymphoma
JT  - Leukemia & lymphoma
JID - 9007422
RN  - 0 (RNA, Neoplasm)
RN  - 0 (Recombinant Proteins)
RN  - 63231-63-0 (RNA)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - *Acridine Orange
MH  - Adult
MH  - Bone Marrow/*pathology
MH  - Cell Cycle/drug effects
MH  - Child
MH  - Flow Cytometry/methods
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - Granulocytes/cytology/pathology
MH  - Humans
MH  - Leukemia, Myeloid, Acute/*blood/*pathology
MH  - Lymphocytes/cytology/pathology
MH  - Monocytes/cytology/pathology
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*blood/*pathology
MH  - RNA/metabolism
MH  - RNA, Neoplasm/metabolism
MH  - Recombinant Proteins/pharmacology
MH  - Reference Values
EDAT- 1994/03/01 00:00
MHDA- 1994/03/01 00:01
CRDT- 1994/03/01 00:00
PHST- 1994/03/01 00:00 [pubmed]
PHST- 1994/03/01 00:01 [medline]
PHST- 1994/03/01 00:00 [entrez]
AID - 10.3109/10428199409051653 [doi]
PST - ppublish
SO  - Leuk Lymphoma. 1994 Mar;13(1-2):61-73. doi: 10.3109/10428199409051653.