PMID- 7517920
OWN - NLM
STAT- MEDLINE
DCOM- 19940809
LR  - 20220409
IS  - 0020-7136 (Print)
IS  - 0020-7136 (Linking)
VI  - 58
IP  - 2
DP  - 1994 Jul 15
TI  - Human astrocytomas and glioblastomas express monocyte chemoattractant protein-1 
      (MCP-1) in vivo and in vitro.
PG  - 240-7
AB  - Expression of the monocyte chemoattractant protein-1 (MCP-1) was examined in 
      human central nervous system tumours (glioblastomas and astrocytomas) and normal 
      human brain. Northern blot analysis demonstrated constitutive expression of MCP-1 
      mRNA in 6 of 12 glioblastoma cell lines. Expression could be stimulated by 
      interleukin (IL)-1 beta and tumour necrosis factor (TNF)-alpha in all cell lines 
      tested. Immunoprecipitation demonstrated secretion of both isoforms, MCP-1 alpha 
      and -beta, of the MCP-1 protein. Reverse-transcription polymerase chain reaction 
      and Northern blot analysis on tissues demonstrated MCP-1 mRNA expression in 17 of 
      17 glioblastomas, 3 of 6 anaplastic astrocytomas and 6 of 6 low-grade 
      astrocytomas, as well as in fetal brain but not in normal adult brain. In situ 
      hybridization on 2 glioblastomas and 1 low-grade astrocytoma indicates that 
      neoplastic astrocytes and endothelial cells express MCP-1 mRNA in vivo. Moreover, 
      tumour cyst fluids of glioblastomas and astrocytomas were able to induce monocyte 
      chemoattraction in an in vitro assay. This chemotactic activity was specifically 
      neutralized by anti-MCP-1 antibodies in 9 of 10 samples, further demonstrating 
      the production of bioactive MCP-1 in vivo and supporting an important role for 
      this factor in the infiltration of monocytes/macrophages into tumour tissue.
FAU - Desbaillets, I
AU  - Desbaillets I
AD  - Laboratory of Tumour Biology and Genetics, University Hospital (CHUV), Lausanne, 
      Switzerland.
FAU - Tada, M
AU  - Tada M
FAU - de Tribolet, N
AU  - de Tribolet N
FAU - Diserens, A C
AU  - Diserens AC
FAU - Hamou, M F
AU  - Hamou MF
FAU - Van Meir, E G
AU  - Van Meir EG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemotactic Factors)
RN  - 0 (Cytokines)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.7.49 (RNA-Directed DNA Polymerase)
SB  - IM
GS  - MCP-1
MH  - Astrocytoma/genetics/*metabolism
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Brain Neoplasms/genetics/*metabolism
MH  - Chemokine CCL2
MH  - Chemotactic Factors/*biosynthesis/genetics/metabolism
MH  - Cytokines/biosynthesis/genetics/metabolism
MH  - Gene Expression
MH  - Glioblastoma/genetics/*metabolism
MH  - Humans
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/genetics
MH  - RNA-Directed DNA Polymerase
MH  - Tumor Cells, Cultured
EDAT- 1994/07/15 00:00
MHDA- 1994/07/15 00:01
CRDT- 1994/07/15 00:00
PHST- 1994/07/15 00:00 [pubmed]
PHST- 1994/07/15 00:01 [medline]
PHST- 1994/07/15 00:00 [entrez]
AID - 10.1002/ijc.2910580216 [doi]
PST - ppublish
SO  - Int J Cancer. 1994 Jul 15;58(2):240-7. doi: 10.1002/ijc.2910580216.