PMID- 7520087
OWN - NLM
STAT- MEDLINE
DCOM- 19940915
LR  - 20061115
IS  - 0360-4012 (Print)
IS  - 0360-4012 (Linking)
VI  - 38
IP  - 1
DP  - 1994 May 1
TI  - Changes of beta-amyloid precursor protein splice patterns in brain cell aggregate 
      cultures.
PG  - 32-40
AB  - The splice pattern of beta-amyloid precursor protein (beta-APP) has been studied 
      in a variety of neuronal and glial cells and in brain cell aggregate cultures by 
      the polymerase chain reaction (PCR). The brain-typical pattern, in which 
      beta-APP695 is the dominant form, has been found only in aggregate cultures but 
      not in any of the other cell types including neuronal cell lines. Selective 
      elimination of glial cells from aggregates resulted in increased quantities of 
      beta-APP695, whereas removal of neurons led to a reduction of beta-APP695 and to 
      an elevation of beta-APP751 and beta-APP770. This shift of splice pattern was not 
      observed in cocultures of the neuronal cell line PC 12 with primary astrocytes 
      combined in a variety of cellular ratios. Blood serum, which is an essential 
      component of these cultures, tested on aggregates, did not reduce the amount of 
      beta-APP695 or have any marked effects on splice patterns generally. From these 
      results it is concluded that investigations on brain-typical splicing of beta-APP 
      require primary neurons. Neuronal cell lines may be no suitable model systems. 
      Splicing events favoring production of beta-APP695 may mark an important, very 
      early step of amyloid formation in the brain.
FAU - Gebicke-Haerter, P J
AU  - Gebicke-Haerter PJ
AD  - Department of Psychiatry, University of Freiburg Medical School, Germany.
FAU - Appel, K
AU  - Appel K
FAU - Honegger, P
AU  - Honegger P
FAU - Berger, M
AU  - Berger M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Amyloid beta-Protein Precursor)
RN  - 0 (Culture Media, Conditioned)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Amyloid beta-Protein Precursor/*metabolism
MH  - Animals
MH  - Base Sequence
MH  - Brain/*cytology
MH  - Brain Chemistry/*physiology
MH  - Cell Aggregation
MH  - Cells, Cultured
MH  - Culture Media, Conditioned
MH  - Molecular Sequence Data
MH  - Neuroglia/metabolism
MH  - Neurons/metabolism
MH  - PC12 Cells
MH  - Polymerase Chain Reaction
MH  - RNA/biosynthesis
MH  - Rats
EDAT- 1994/05/01 00:00
MHDA- 1994/05/01 00:01
CRDT- 1994/05/01 00:00
PHST- 1994/05/01 00:00 [pubmed]
PHST- 1994/05/01 00:01 [medline]
PHST- 1994/05/01 00:00 [entrez]
AID - 10.1002/jnr.490380106 [doi]
PST - ppublish
SO  - J Neurosci Res. 1994 May 1;38(1):32-40. doi: 10.1002/jnr.490380106.