PMID- 7520777
OWN - NLM
STAT- MEDLINE
DCOM- 19940928
LR  - 20220409
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 84
IP  - 5
DP  - 1994 Sep 1
TI  - Tumor necrosis factor-alpha (TNF-alpha) potently enhances in vitro macrophage 
      production from primitive murine hematopoietic progenitor cells in combination 
      with stem cell factor and interleukin-7: novel stimulatory role of p55 TNF 
      receptors.
PG  - 1528-33
AB  - Tumor necrosis factor-alpha (TNF-alpha) is a bifunctional regulator of 
      hematopoiesis, and its cellular responses are mediated by two distinct cell 
      surface receptors. TNF-alpha generally inhibits the growth of primitive murine 
      hematopoietic progenitor cells (Lin-Scal+) in response to multiple cytokine 
      combinations, and the p75 TNF receptor is essential in signaling such inhibition. 
      In the present study we show the reverse phenomenon in that TNF-alpha on the same 
      progenitor cell population in combination with stem cell factor (SCF) and 
      interleukin-7 (IL-7) through the p55 TNF receptor can recruit additional 
      progenitors to proliferate. In contrast, TGF-beta 1, another bifunctional 
      regulator of hematopoietic progenitor cell growth, completely blocked SCF plus 
      IL-7-induced proliferation. TNF-alpha increased the number of responding 
      progenitors, as well as the size of the colonies formed. The synergistic effects 
      of TNF-alpha were seen at the single cell level, suggesting that its effects are 
      directly mediated. Finally, whereas SCF plus IL-7 promoted primarily 
      granulopoiesis, the addition of TNF-alpha switched the differentiation toward the 
      production of almost exclusively macrophages.
FAU - Fahlman, C
AU  - Fahlman C
AD  - Department of Immunology, Norwegian Radium Hospital, Montebello.
FAU - Jacobsen, F W
AU  - Jacobsen FW
FAU - Veiby, O P
AU  - Veiby OP
FAU - McNiece, I K
AU  - McNiece IK
FAU - Blomhoff, H K
AU  - Blomhoff HK
FAU - Jacobsen, S E
AU  - Jacobsen SE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Hematopoietic Cell Growth Factors)
RN  - 0 (Interleukin-7)
RN  - 0 (Receptors, Tumor Necrosis Factor)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Stem Cell Factor)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells
MH  - Cell Adhesion Molecules/pharmacology
MH  - Cell Differentiation/*drug effects
MH  - Cell Division/drug effects
MH  - Cells, Cultured
MH  - Dose-Response Relationship, Drug
MH  - Drug Synergism
MH  - Hematopoietic Cell Growth Factors/*pharmacology
MH  - Hematopoietic Stem Cells/*cytology/drug effects
MH  - Humans
MH  - Interleukin-7/*pharmacology
MH  - Kinetics
MH  - Macrophages/*cytology/drug effects
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Rats
MH  - Receptors, Tumor Necrosis Factor/drug effects/*physiology
MH  - Recombinant Proteins/pharmacology
MH  - Stem Cell Factor
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 1994/09/01 00:00
MHDA- 1994/09/01 00:01
CRDT- 1994/09/01 00:00
PHST- 1994/09/01 00:00 [pubmed]
PHST- 1994/09/01 00:01 [medline]
PHST- 1994/09/01 00:00 [entrez]
AID - S0006-4971(20)77622-6 [pii]
PST - ppublish
SO  - Blood. 1994 Sep 1;84(5):1528-33.