PMID- 7520867
OWN - NLM
STAT- MEDLINE
DCOM- 19940929
LR  - 20220511
IS  - 0261-4189 (Print)
IS  - 1460-2075 (Electronic)
IS  - 0261-4189 (Linking)
VI  - 13
IP  - 16
DP  - 1994 Aug 15
TI  - PTP-PEST: a protein tyrosine phosphatase regulated by serine phosphorylation.
PG  - 3763-71
AB  - The protein tyrosine phosphatase PTP-PEST is an 88 kDa cytosolic enzyme which is 
      ubiquitously expressed in mammalian tissues. We have expressed PTP-PEST using 
      recombinant baculovirus, and purified the protein essentially to homogeneity in 
      order to investigate phosphorylation as a potential mechanism of regulation of 
      the enzyme. PTP-PEST is phosphorylated in vitro by both cyclic AMP-dependent 
      protein kinase (PKA) and protein kinase C (PKC) at two major sites, which we have 
      identified as Ser39 and Ser435. PTP-PEST is also phosphorylated on both Ser39 and 
      Ser435 following treatment of intact HeLa cells with TPA, forskolin or isobutyl 
      methyl xanthine (IBMX). Phosphorylation of Ser39 in vitro decreases the activity 
      of PTP-PEST by reducing its affinity for substrate. In addition, PTP-PEST 
      immunoprecipitated from TPA-treated cells displayed significantly lower PTP 
      activity than enzyme obtained from untreated cells. Our results suggest that both 
      PKC and PKA are capable of phosphorylating, and therefore inhibiting, PTP-PEST in 
      vivo, offering a mechanism whereby signal transduction pathways acting through 
      either PKA or PKC may directly influence cellular processes involving reversible 
      tyrosine phosphorylation.
FAU - Garton, A J
AU  - Garton AJ
AD  - Cold Spring Harbor Laboratory, NY 11724-2208.
FAU - Tonks, N K
AU  - Tonks NK
LA  - eng
GR  - CA53840/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (Phorbol Esters)
RN  - 0 (Phosphopeptides)
RN  - 0 (Recombinant Proteins)
RN  - 1F7A44V6OU (Colforsin)
RN  - 452VLY9402 (Serine)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - EC 3.1.3.48 (PTPN12 protein, human)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatase, Non-Receptor Type 12)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatases)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
RN  - TBT296U68M (1-Methyl-3-isobutylxanthine)
SB  - IM
MH  - 1-Methyl-3-isobutylxanthine/pharmacology
MH  - Baculoviridae/genetics
MH  - Colforsin/pharmacology
MH  - Cyclic AMP-Dependent Protein Kinases/metabolism
MH  - *Gene Expression Regulation, Enzymologic
MH  - HeLa Cells/enzymology/metabolism
MH  - Humans
MH  - Peptide Mapping
MH  - Phorbol Esters
MH  - Phosphopeptides/analysis
MH  - Phosphorylation/drug effects
MH  - Protein Kinase C/metabolism
MH  - Protein Tyrosine Phosphatase, Non-Receptor Type 12
MH  - Protein Tyrosine Phosphatases/genetics/*metabolism
MH  - Recombinant Proteins/metabolism
MH  - Sequence Analysis
MH  - Serine/metabolism
MH  - Tetradecanoylphorbol Acetate/pharmacology
PMC - PMC395289
EDAT- 1994/08/15 00:00
MHDA- 1994/08/15 00:01
PMCR- 1995/08/15
CRDT- 1994/08/15 00:00
PHST- 1994/08/15 00:00 [pubmed]
PHST- 1994/08/15 00:01 [medline]
PHST- 1994/08/15 00:00 [entrez]
PHST- 1995/08/15 00:00 [pmc-release]
AID - 10.1002/j.1460-2075.1994.tb06687.x [doi]
PST - ppublish
SO  - EMBO J. 1994 Aug 15;13(16):3763-71. doi: 10.1002/j.1460-2075.1994.tb06687.x.