PMID- 7520954
OWN - NLM
STAT- MEDLINE
DCOM- 19940927
LR  - 20190725
IS  - 0085-2538 (Print)
IS  - 0085-2538 (Linking)
VI  - 45
IP  - 5
DP  - 1994 May
TI  - Albumin and transferrin synthesis are increased in H4 cells by serum from 
      analbuminemic or nephrotic rats.
PG  - 1381-7
AB  - The hepatic synthesis of several proteins, including both albumin and transferrin 
      is increased in the nephrotic syndrome. While active suppression of albumin 
      synthesis by lymphokines has been described, it has been assumed that 
      augmentation of albumin synthesis is governed by a physical factor, plasma 
      oncotic pressure (pi), and that this regulation is by a direct effect of pi on 
      hepatocytes. The mechanisms have not been defined. Furthermore, experiments 
      relying on suppression of protein synthesis may only test non-specific inhibitory 
      effects of the experimental intervention. We tested an alternative hypothesis 
      that a serum factor(s) present in hypooncotic states stimulates albumin 
      synthesis. We incubated an immortalized cell line derived from rat hepatocytes 
      (H4 cells) with serum from Nagase analbuminemic rats (NAR) and rats with passive 
      Heymann nephritis (HN), a model of the nephrotic syndrome. Synthesis 
      (incorporation of [35S]methionine) into both albumin and transferrin was 
      increased significantly. The stimulatory effect of these sera was not 
      extinguished by addition of rat or human albumin to the medium prior to or during 
      incubation, even when pi in the incubation medium was increased to normal plasma 
      levels by added albumin. Incorporation of [35S]methionine into albumin was 7841 
      +/- 394 cpm/mg cell protein using 10% NAR serum in the presence of human albumin 
      (medium pi 26.1 +/- 0.17) versus 5149 +/- 420 cpm incorporation (P < 0.05) in the 
      presence of control serum and in the absence of added albumin (medium pi 2.06 +/- 
      0.26 mm Hg, P < 0.001). The stimulatory activity was preserved following heating 
      of serum for one hour at 60 degrees C.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Sun, X
AU  - Sun X
AD  - Department of Medicine, University of California Davis School of Medicine.
FAU - Kaysen, G A
AU  - Kaysen GA
LA  - eng
GR  - NIDDK 42297/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Kidney Int
JT  - Kidney international
JID - 0323470
RN  - 0 (Biological Factors)
RN  - 0 (Blood Proteins)
RN  - 0 (Serum Albumin)
RN  - 0 (Transferrin)
RN  - 63231-63-0 (RNA)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Biological Factors/pharmacology
MH  - Blood Proteins/pharmacology
MH  - Cell Line
MH  - DNA/biosynthesis
MH  - Glomerulonephritis/blood
MH  - Liver/cytology/*metabolism
MH  - Male
MH  - Nephrotic Syndrome/*blood
MH  - RNA/biosynthesis
MH  - Rats
MH  - Rats, Mutant Strains
MH  - Rats, Sprague-Dawley
MH  - Serum Albumin/*biosynthesis/*deficiency
MH  - Transferrin/*biosynthesis
EDAT- 1994/05/01 00:00
MHDA- 1994/05/01 00:01
CRDT- 1994/05/01 00:00
PHST- 1994/05/01 00:00 [pubmed]
PHST- 1994/05/01 00:01 [medline]
PHST- 1994/05/01 00:00 [entrez]
AID - S0085-2538(15)58461-5 [pii]
AID - 10.1038/ki.1994.180 [doi]
PST - ppublish
SO  - Kidney Int. 1994 May;45(5):1381-7. doi: 10.1038/ki.1994.180.