PMID- 7521336
OWN - NLM
STAT- MEDLINE
DCOM- 19941004
LR  - 20061115
IS  - 0021-9304 (Print)
IS  - 0021-9304 (Linking)
VI  - 28
IP  - 3
DP  - 1994 Mar
TI  - Factor XII fragment and kallikrein generation in plasma during incubation with 
      biomaterials.
PG  - 349-52
AB  - Blood biocompatibility of medical devices is in many ways dependent on surface 
      characteristics and biochemical blood material interactions. In this study, the 
      contact system, in which the activation of factor XII and plasma kallikrein is 
      included, is highlighted. This article describes a simple chromogenic assay to 
      determine the Hageman Factor fragment (HFf, or factor XIIf) and kallikrein 
      activity in vitro. The assay is based on conversion of Z-Lys-Phe-Arg-pNA.2HCl to 
      which human factor XIIf and kallikrein appeared to have a high affinity. To 
      discriminate between the serine proteases factor XIIf and kallikrein to cleave 
      this substrate, aprotinin was added to one of two complementary samples. In this 
      in vitro study, standardized disks from glass, high-density polyethylene (HDPE), 
      polytetrafluoro ethylene (PTFE), and polydimethyl siloxane (PDMS) were studied 
      for their capacity to generate factor XIIf and kallikrein in plasma. Kaolin was 
      used as positive control. On glass disks the highest and on HDPE the lowest 
      generation of factor XIIf and kallikrein were found, both with a ratio of 1:1. On 
      PDMS and on PTFE disks protease activities were intermediate, but with a factor 
      XIIf and kallikrein activity ratio of 1:2 and 1:4, respectively. Apparently 
      because of the hydrophobic surface character of PDMS and PTFE, these surfaces 
      absorb or fail to produce the factor XIIf. This assay appeared to be 
      discriminative even for materials that are considered mild activators of the 
      contact system and can therefore be used as a standard method to qualify 
      biomaterials.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - van der Kamp, K W
AU  - van der Kamp KW
AD  - BioMedical Technology Center/Thorax Center, University Hospital Groningen, The 
      Netherlands.
FAU - van Oeveren, W
AU  - van Oeveren W
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - J Biomed Mater Res
JT  - Journal of biomedical materials research
JID - 0112726
RN  - 0 (Biocompatible Materials)
RN  - 0 (Chromogenic Compounds)
RN  - 0 (Oligopeptides)
RN  - 0 (Peptide Fragments)
RN  - 0 (Polyethylenes)
RN  - 0 (Silicone Elastomers)
RN  - 0 (benzyloxycarbonyl-lysyl-phenylalanyl-arginine-4-nitroanilide)
RN  - 9001-30-3 (Factor XII)
RN  - 9002-84-0 (Polytetrafluoroethylene)
RN  - 9087-70-1 (Aprotinin)
RN  - EC 3.4.21.- (Kallikreins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Aprotinin/pharmacology
MH  - Biocompatible Materials/*chemistry
MH  - *Blood Coagulation
MH  - Chromogenic Compounds/metabolism
MH  - Enzyme Activation
MH  - Factor XII/*metabolism
MH  - *Fibrinolysis
MH  - Glass/chemistry
MH  - Humans
MH  - Kallikreins/*biosynthesis
MH  - Materials Testing
MH  - Molecular Sequence Data
MH  - Oligopeptides/metabolism
MH  - Peptide Fragments/*blood
MH  - Polyethylenes/chemistry
MH  - Polytetrafluoroethylene/chemistry
MH  - Silicone Elastomers/chemistry
MH  - Substrate Specificity
MH  - Surface Properties
EDAT- 1994/03/01 00:00
MHDA- 1994/03/01 00:01
CRDT- 1994/03/01 00:00
PHST- 1994/03/01 00:00 [pubmed]
PHST- 1994/03/01 00:01 [medline]
PHST- 1994/03/01 00:00 [entrez]
AID - 10.1002/jbm.820280309 [doi]
PST - ppublish
SO  - J Biomed Mater Res. 1994 Mar;28(3):349-52. doi: 10.1002/jbm.820280309.