PMID- 7521532
OWN - NLM
STAT- MEDLINE
DCOM- 19941004
LR  - 20201209
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 91
IP  - 18
DP  - 1994 Aug 30
TI  - A small mitochondrial double-stranded (ds) RNA element associated with a 
      hypovirulent strain of the chestnut blight fungus and ancestrally related to 
      yeast cytoplasmic T and W dsRNAs.
PG  - 8680-4
AB  - A small double-stranded (ds) RNA element was isolated from a moderately 
      hypovirulent strain of the chestnut blight fungus Cryphonectria parasitica 
      (Murr.) Barr. from eastern New Jersey. Virulence was somewhat lower in the 
      dsRNA-containing strain than in a virulent dsRNA-free control strain, but colony 
      morphology and sporulation levels were comparable. A library of cDNA clones was 
      constructed, and overlapping clones representing the entire genome were 
      sequenced. The 2728-bp dsRNA was considerably smaller than previously 
      characterized C. parasitica dsRNAs, which are 12-13 kb and ancestrally related to 
      the Potyviridae family of plant viruses. Sequence analysis revealed one large 
      open reading frame, but only if mitochondrial codon usage (UGA = Trp) was 
      invoked. Nuclease assays of purified mitochondria confirmed that the dsRNA was 
      localized within mitochondria. Assuming mitochondrial translation, the deduced 
      amino acid sequence had landmarks typical of RNA-dependent RNA polymerases. 
      Alignments of the conserved regions indicate that this dsRNA is more closely 
      related to yeast T and W dsRNAs and single-stranded RNA bacteriophages such as Q 
      beta than to other hypovirulence-associated dsRNAs.
FAU - Polashock, J J
AU  - Polashock JJ
AD  - Department of Plant Pathology, Rutgers University, New Brunswick, NJ 08903.
FAU - Hillman, B I
AU  - Hillman BI
LA  - eng
SI  - GENBANK/L31849
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (RNA, Double-Stranded)
RN  - 0 (RNA, Fungal)
RN  - 0 (RNA, Mitochondrial)
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Structural Proteins)
RN  - 63231-63-0 (RNA)
RN  - EC 2.7.7.48 (RNA-Dependent RNA Polymerase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Ascomycota/*genetics/pathogenicity
MH  - Base Sequence
MH  - *Genes, Viral
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Plant Diseases/microbiology
MH  - Plant Viruses/*genetics
MH  - RNA/genetics
MH  - RNA, Double-Stranded/*genetics
MH  - RNA, Fungal/*genetics
MH  - RNA, Mitochondrial
MH  - RNA, Viral/*genetics
MH  - RNA-Dependent RNA Polymerase/genetics
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Trees
MH  - Viral Structural Proteins/*genetics
PMC - PMC44670
EDAT- 1994/08/30 00:00
MHDA- 1994/08/30 00:01
PMCR- 1995/02/28
CRDT- 1994/08/30 00:00
PHST- 1994/08/30 00:00 [pubmed]
PHST- 1994/08/30 00:01 [medline]
PHST- 1994/08/30 00:00 [entrez]
PHST- 1995/02/28 00:00 [pmc-release]
AID - 10.1073/pnas.91.18.8680 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1994 Aug 30;91(18):8680-4. doi: 
      10.1073/pnas.91.18.8680.