PMID- 7522623
OWN - NLM
STAT- MEDLINE
DCOM- 19941107
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 84
IP  - 7
DP  - 1994 Oct 1
TI  - Human interleukin-4 enhances stromal cell-dependent hematopoiesis: costimulation 
      with stem cell factor.
PG  - 2189-96
AB  - Interleukin-4 (IL-4) has distinct hematopoietic activities, primarily as a 
      costimulant with other cytokines to enhance colony formation of hematopoietic 
      progenitors. We investigated the influence of IL-4 on stromal cell-supported 
      long-term cultures (LTCs) of normal human bone marrow. Addition of IL-4 to LTCs 
      of unseparated bone marrow or highly enriched CD34+ cells resulted in a 
      significant increase of myeloid progenitors in the nonadherent, as well as in the 
      stromal cell-adherent cell populations. In contrast, the total cell number was 
      not influenced by IL-4, suggesting a selective effect on primitive progenitor 
      cells. Cord blood cells or CD34+ bone marrow cells were incubated with stem cell 
      factor (SCF) and/or IL-4 in stromal cell-free cultures. In these experiments, a 
      twofold to fivefold increase of myeloid progenitor cells was observed in the 
      presence of SCF and IL-4 as compared with SCF alone. Preincubation of the stromal 
      cell cultures with IL-4 resulted in an enhanced adherence of CD34+ cells to the 
      stromal layer. Secretion of hematopoietic growth factors produced by the stromal 
      cells, such as granulocyte-macrophage colony-stimulating factor (G-CSF), and 
      IL-1, was inhibited by IL-4. Thus, the increase of hematopoietic progenitors in 
      LTCs, as observed in the presence of IL-4, can be at least partially explained by 
      a costimulation of SCF and IL-4 on primitive progenitor cells and by an 
      enhancement of hematopoietic cells to stroma. The downregulation of CSFs by IL-4 
      might prevent the expansion of the mature hematopoietic cell compartment.
FAU - Keller, U
AU  - Keller U
AD  - Department of Medicine, Johannes-Gutenberg-University School of Medicine, Mainz, 
      Germany.
FAU - Aman, M J
AU  - Aman MJ
FAU - Derigs, G
AU  - Derigs G
FAU - Huber, C
AU  - Huber C
FAU - Peschel, C
AU  - Peschel C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, CD34)
RN  - 0 (Cytokines)
RN  - 0 (Hematopoietic Cell Growth Factors)
RN  - 0 (Interleukin-3)
RN  - 0 (Stem Cell Factor)
RN  - 207137-56-2 (Interleukin-4)
SB  - IM
MH  - Antigens, CD/analysis
MH  - Antigens, CD34
MH  - *Bone Marrow Cells
MH  - Cell Adhesion/drug effects
MH  - Colony-Forming Units Assay
MH  - Cytokines/metabolism
MH  - Drug Synergism
MH  - Hematopoiesis/*drug effects
MH  - Hematopoietic Cell Growth Factors/*administration & dosage
MH  - Humans
MH  - In Vitro Techniques
MH  - Interleukin-3/pharmacology
MH  - Interleukin-4/*administration & dosage
MH  - Stem Cell Factor
EDAT- 1994/10/01 00:00
MHDA- 1994/10/01 00:01
CRDT- 1994/10/01 00:00
PHST- 1994/10/01 00:00 [pubmed]
PHST- 1994/10/01 00:01 [medline]
PHST- 1994/10/01 00:00 [entrez]
AID - S0006-4971(20)76500-6 [pii]
PST - ppublish
SO  - Blood. 1994 Oct 1;84(7):2189-96.