PMID- 7522960
OWN - NLM
STAT- MEDLINE
DCOM- 19941103
LR  - 20131121
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 54
IP  - 20
DP  - 1994 Oct 15
TI  - Modulation by retinoic acid (RA) of squamous cell differentiation, cellular 
      RA-binding proteins, and nuclear RA receptors in human head and neck squamous 
      cell carcinoma cell lines.
PG  - 5479-87
AB  - The ability of all-trans-retinoic acid (RA) to modulate the growth and squamous 
      differentiation of four head and neck squamous cell carcinoma cell lines (183, 
      886, 1483, and SqCC/Y1) was examined, and the relationship of their state of 
      squamous differentiation and RA responsiveness to the expression of cytosolic 
      RA-binding proteins (CRABPs), nuclear RA receptors (RARs), and retinoid X 
      receptors (RXRs) was investigated. RA inhibited proliferation of all but the 183 
      cell line and suppressed squamous differentiation markers K1 keratin, type 1 
      transglutaminase, and involucrin mRNAs and proteins to varying degrees in 183, 
      1483, and SqCC/Y1 cells. Traces of CRABP-I mRNA were detected only in the 886 
      cells, whereas CRABP-II mRNA was detected in the other three cell lines. RA 
      suppressed CRABP-II expression in SqCC/Y1 cells but had no effect on its 
      expression in the other cell lines. All cell lines expressed mRNAs for RAR-alpha, 
      RAR-beta, RAR-gamma, and RXR-alpha. The RAR-beta mRNA level was lowest in the 
      SqCC/Y1 cells, and RXR-beta and RXR-gamma were not detected in any of the cell 
      lines. RA treatment increased the levels of the three RAR mRNAs in most of the 
      cell lines but had no effect on the RXR mRNAs. The CRABP-II mRNA level in SqCC/Y1 
      cells was lowest in cells grown in serum-free medium and increased when the cells 
      were grown in medium with 5 or 10% serum. In contrast, the RXR-alpha mRNA level 
      was inversely related to serum concentration. The results show that, in head and 
      neck squamous cell carcinoma cells, there are no simple relationships among the 
      expression of CRABPs, RARs, and RXRs and either squamous differentiation or 
      response to RA-induced growth inhibition or suppression of squamous 
      differentiation.
FAU - Zou, C P
AU  - Zou CP
AD  - Department of Tumor Biology, University of Texas M. D. Anderson Cancer Center, 
      Houston 77030.
FAU - Clifford, J L
AU  - Clifford JL
FAU - Xu, X C
AU  - Xu XC
FAU - Sacks, P G
AU  - Sacks PG
FAU - Chambon, P
AU  - Chambon P
FAU - Hong, W K
AU  - Hong WK
FAU - Lotan, R
AU  - Lotan R
LA  - eng
GR  - CA57166/CA/NCI NIH HHS/United States
GR  - P01 CA52051/CA/NCI NIH HHS/United States
GR  - R25 CA57730/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
RN  - 0 (Protein Precursors)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Retinoic Acid)
RN  - 0 (retinoic acid binding protein II, cellular)
RN  - 5688UTC01R (Tretinoin)
RN  - 60108-77-2 (involucrin)
RN  - 68238-35-7 (Keratins)
RN  - EC 2.3.2.13 (Transglutaminases)
SB  - IM
MH  - Carcinoma, Squamous Cell/*metabolism/*pathology
MH  - Cell Differentiation/drug effects
MH  - Head and Neck Neoplasms/*metabolism/*pathology
MH  - Humans
MH  - Keratins/*metabolism
MH  - Protein Precursors/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Receptors, Retinoic Acid/*metabolism
MH  - Transglutaminases/*metabolism
MH  - Tretinoin/*pharmacology
MH  - Tumor Cells, Cultured
EDAT- 1994/10/15 00:00
MHDA- 1994/10/15 00:01
CRDT- 1994/10/15 00:00
PHST- 1994/10/15 00:00 [pubmed]
PHST- 1994/10/15 00:01 [medline]
PHST- 1994/10/15 00:00 [entrez]
PST - ppublish
SO  - Cancer Res. 1994 Oct 15;54(20):5479-87.