PMID- 7523165
OWN - NLM
STAT- MEDLINE
DCOM- 19941110
LR  - 20061115
IS  - 0301-472X (Print)
IS  - 0301-472X (Linking)
VI  - 22
IP  - 12
DP  - 1994 Nov
TI  - Basic fibroblast growth factor increases retroviral-mediated gene transfer into 
      human hematopoietic peripheral blood progenitor cells.
PG  - 1129-33
AB  - Retroviral vector-mediated gene transfer into human hematopoietic stem cells 
      (HSC) may permit gene therapy of certain genetic diseases. Stimulation of HSC 
      with hematopoietic growth factors (GF) has been shown to increase the level of 
      retroviral transduction. We have studied the effect of basic fibroblast growth 
      factor (bFGF), alone and in combination with other GFs, on the efficiency of 
      transfer of the bacterial neomycin phosphotransferase (neoR) gene into human 
      CD34(+)-enriched peripheral blood hematopoietic progenitor cells. The combination 
      of bFGF, interleukin-3 (IL-3), IL-6, and stem cell factor (SCF) resulted in a 
      transduction efficiency of 37 and 35% for G418-resistant colony-forming 
      units-granulocyte/macrophage (CFU-GM) and mixed colonies multipotent 
      colony-forming units (CFU-GEMM), respectively, which was significantly higher 
      than the corresponding figures obtained with IL-3, IL-6, and SCF. The optimal 
      concentration of bFGF was between 20 and 200 ng/mL. bFGF alone had no effect on 
      the transduction rate. These results indicated a synergism in the action of bFGF, 
      IL-3, IL-6, and SCF to enhance gene transduction rates into human hematopoietic 
      progenitor cells.
FAU - Dilber, M S
AU  - Dilber MS
AD  - Department of Medicine, Karolinska Institute, Huddinge Hospital, Sweden.
FAU - Bjorkstrand, B
AU  - Bjorkstrand B
FAU - Li, K J
AU  - Li KJ
FAU - Smith, C I
AU  - Smith CI
FAU - Xanthopoulos, K G
AU  - Xanthopoulos KG
FAU - Gahrton, G
AU  - Gahrton G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Exp Hematol
JT  - Experimental hematology
JID - 0402313
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, CD34)
RN  - 0 (Hematopoietic Cell Growth Factors)
RN  - 0 (Interleukin-3)
RN  - 0 (Interleukin-6)
RN  - 0 (Stem Cell Factor)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - EC 2.7.1.- (Phosphotransferases (Alcohol Group Acceptor))
RN  - EC 2.7.1.95 (Kanamycin Kinase)
SB  - IM
MH  - Antigens, CD/analysis
MH  - Antigens, CD34
MH  - Drug Interactions
MH  - Fibroblast Growth Factor 2/administration & dosage/*pharmacology
MH  - *Gene Transfer Techniques
MH  - Granulocytes/metabolism
MH  - Hematopoietic Cell Growth Factors/pharmacology
MH  - Hematopoietic Stem Cells/*metabolism
MH  - Humans
MH  - Interleukin-3/pharmacology
MH  - Interleukin-6/pharmacology
MH  - Kanamycin Kinase
MH  - Macrophages/metabolism
MH  - Phosphotransferases (Alcohol Group Acceptor)/genetics
MH  - Retroviridae/*genetics
MH  - Stem Cell Factor
EDAT- 1994/11/01 00:00
MHDA- 1994/11/01 00:01
CRDT- 1994/11/01 00:00
PHST- 1994/11/01 00:00 [pubmed]
PHST- 1994/11/01 00:01 [medline]
PHST- 1994/11/01 00:00 [entrez]
PST - ppublish
SO  - Exp Hematol. 1994 Nov;22(12):1129-33.